Single molecule studies of helicases with magnetic tweezers
- Hodeib, Samar and Raj, Saurabh and Manosas, M. and Zhang, Weiting and Bagchi, Debjani and Ducos, Bertrand and Allemand, Jean-Francois and Bensimon, David and Croquette, VincentMETHODS 105
, 3-15 (2016)
Abstract : Helicases are a broad family of enzymes that perform crucial functions
in DNA replication and in the maintenance of DNA and RNA integrity. A
detailed mechanical study of helicases on DNA and RNA is possible using
single molecule manipulation methods. Among those, magnetic tweezers (or
traps) present a convenient, moderate throughput assay (tens of enzymes
can be monitored simultaneously) that allow for high resolution (single
base-pair) studies of these enzymes in various conditions and on various
substrates (double and single stranded DNA and RNA). Here we discuss
various implementation of the basic assay relevant for these studies.
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