laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
Jean-François ALLEMAND 


MOLECULAR CELL 


1
P U B L I C A T I O N S



 
2013
RecA-Promoted, RecFOR-Independent Progressive Disassembly of Replisomes Stalled by Helicase Inactivation (Retracted article. See vol. 54, pg. 537, 2014) - Lia, Giuseppe and Rigato, Annafrancesca and Long, Emilie and Chagneau, Carine and Le Masson, Marie and Allemand, Jean-Francois and Michel, Benedicte
MOLECULAR CELL 49547-557 (2013) 
LPS


Abstract : In all organisms, replication impairment is a recognized source of genomic instability, raising an increasing interest in the fate of inactivated replication forks. We used Escherichia coli strains with a temperature-inactivated replicative helicase (DnaB) and in vivo single-molecule microscopy to quantify the detailed molecular processing of stalled replication forks. After helicase inactivation, RecA binds to blocked replication forks and is essential for the rapid release of hPol III. The entire holoenzyme is disrupted little by little, with some components lost in few minutes, while others are stable in 70\% of cells for at least 1 hr. Although replisome dissociation is delayed in a recA mutant, it is not affected by RecF or RecO inactivation. RecFOR are required for full RecA filaments formation, and we propose that polymerase clearance can be catalyzed by short, RecFOR-independent RecA filaments. Our results identify a function for the universally conserved, central recombination protein RecA.