laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
Jean-François ALLEMAND 


SCIENCE 


3
P U B L I C A T I O N S



 
2014
1
Polymerase exchange during Okazaki fragment synthesis observed in living cells (Retraction of vol 335, pg 328, 2012) - Lia, Giuseppe and Michel, Benedicte and Allemand, Jean-Francois
SCIENCE 3461466 (2014) 
LPS
 
2012
Polymerase Exchange During Okazaki Fragment Synthesis Observed in Living Cells (Retracted, see vol 346, pg 1466, 2014) - Lia, Giuseppe and Michel, Benedicte and Allemand, Jean-Francois
SCIENCE 335328-331 (2012) 
LPS


Abstract : DNA replication machineries have been studied extensively, but the kinetics of action of their components remains largely unknown. We report a study of DNA synthesis during replication in living Escherichia coli cells. Using single-molecule microscopy, we observed repetitive fluorescence bursts of single polymerase IIIs (Pol IIIs), indicating polymerase exchange at the replication fork. Fluctuations in the amount of DNA-bound single-stranded DNA-binding protein (SSB) reflect different speeds for the leading-and lagging-strand DNA polymerases. Coincidence analyses of Pol III and SSB fluctuations show that they correspond to the lagging-strand synthesis and suggest the use of a new Pol III for each Okazaki fragment. Based on exchanges involving two Pol IIIs, we propose that the third polymerase in the replisome is involved in lagging-strand synthesis.
 
2007
The manipulation of single biomolecules - Allemand, Jean-Francois and Charvin, Gilles and Croquette, Vincent and Lia, Giuseppe and Bensimon, David
INTERDISCIPLINARY SCIENCE REVIEWS 32149-161 (2007) 
LPS


Abstract : By monitoring the response of individual protein and DNA molecules to pulling and twisting, biophysicists can learn much about their structure and their interactions. In this review we aim to give a flavour of the kinds of investigations that single-molecule techniques have made possible.