laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
David BENSIMON 


CHEMISTRY LETTERS 


1
P U B L I C A T I O N S



 
2009
Single-molecule Visualization of Binding Modes of Helicase to DNA on PEGylated Surfaces - Yokota, Hiroaki and Han, Yong-Woon and Allemand, Jean-Francois and Xi, Xu Guang and Bensimon, David and Croquette, Vincent and Ito, Yoshihiro and Harada, Yoshie
CHEMISTRY LETTERS 38308-309 (2009) 
LPS


Abstract : Binding modes of helicase to various DNA substrates were visualized by single-molecule fluorescence imaging on silicate coverslips coated with poly(ethylene glycol) (PEG) which minimizes the background noise from nonspecific protein adsorption. The results clearly show that the helicase has higher affinity for double-stranded DNA (dsDNA) with a single-stranded DNA (ssDNA) tail than that without one and that multiple helicases can bind to 4.7-kilonucleotide (knt) ssDNA. The study reported here will enhance the capabilities of single-molecule fluorescence studies on DNA-protein interactions.