laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
Bertrand DUCOS 


2
P U B L I C A T I O N S

S E L E C T I O N N E R
P A R M I :




 
2016
Single molecule studies of helicases with magnetic tweezers - Hodeib, Samar and Raj, Saurabh and Manosas, M. and Zhang, Weiting and Bagchi, Debjani and Ducos, Bertrand and Allemand, Jean-Francois and Bensimon, David and Croquette, Vincent
METHODS 1053-15 (2016) 
LPS


Abstract : Helicases are a broad family of enzymes that perform crucial functions in DNA replication and in the maintenance of DNA and RNA integrity. A detailed mechanical study of helicases on DNA and RNA is possible using single molecule manipulation methods. Among those, magnetic tweezers (or traps) present a convenient, moderate throughput assay (tens of enzymes can be monitored simultaneously) that allow for high resolution (single base-pair) studies of these enzymes in various conditions and on various substrates (double and single stranded DNA and RNA). Here we discuss various implementation of the basic assay relevant for these studies. (C) 2016 Elsevier Inc. All rights reserved.
 
2015
Impaired PRC2 activity promotes transcriptional instability and favors breast tumorigenesis - Wassef, Michel and Rodilla, Veronica and Teissandier, Aurelie and Zeitouni, Bruno and Gruel, Nadege and Sadacca, Benjamin and Irondelle, Marie and Charruel, Margaux and Ducos, Bertrand and Michaud, Audrey and Caron, Matthieu and Marangoni, Elisabetta and Chavrier, Philippe and Le Tourneau, Christophe and Kamal, Maud and Pasmant, Eric and Vidaud, Michel and Servant, Nicolas and Reyal, Fabien and Meseure, Dider and Vincent-Salomon, Anne and Fre, Silvia and Margueron, Raphael
GENES \& DEVELOPMENT 292547-2562 (2015) 
LPS


Abstract : Alterations of chromatin modifiers are frequent in cancer, but their functional consequences often remain unclear. Focusing on the Polycomb protein EZH2 that deposits the H3K27me3 (trimethylation of Lys27 of histone H3) mark, we showed that its high expression in solid tumors is a consequence, not a cause, of tumorigenesis. In mouse and human models, EZH2 is dispensable for prostate cancer development and restrains breast tumorigenesis. High EZH2 expression in tumors results from a tight coupling to proliferation to ensure H3K27me3 homeostasis. However, this process malfunctions in breast cancer. Low EZH2 expression relative to proliferation and mutations in Polycomb genes actually indicate poor prognosis and occur in metastases. We show that while altered EZH2 activity consistently modulates a subset of its target genes, it promotes a wider transcriptional instability. Importantly, transcriptional changes that are consequences of EZH2 loss are predominantly irreversible. Our study provides an unexpected understanding of EZH2's contribution to solid tumors with important therapeutic implications.