laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

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ACS CHEMICAL BIOLOGY 


1
P U B L I C A T I O N S



 
2008
Sensing single base incorporation with nanopore micromanipulation - Croquette, Vincent
ACS CHEMICAL BIOLOGY 392-94 (2008)

Abstract : Using single-molecule techniques, scientists can routinely investigate the action of a single enzyme. The goal of such studies is usually to gain accurate information unachievable in ensemble assays, such as the maximum instantaneous rate of reaction, the existence of pauses or backward steps, etc. In the article discussed here, the authors have increased their experimental sensitivity so that they can detect a single enzymatic cycle. This improvement makes possible the use of a polymerase enzyme to sequence a single DNA molecule.