laboratoire de physique statistique
 
 
laboratoire de physique statistique

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ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 


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2015
Accelerating SNARE-Mediated Membrane Fusion by DNA-Lipid Tethers - Xu, Weiming and Wang, Jing and Rothman, James E. and Pincet, Frederic
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 5414388-14392 (2015)

Abstract : SNARE proteins are the core machinery to drive fusion of a vesicle with its target membrane. Inspired by the tethering proteins that bridge the membranes and thus prepare SNAREs for docking and fusion, we developed a lipid-conjugated ssDNA mimic that is capable of regulating SNARE function, in situ. The DNA-lipid tethers consist of a 21 base pairs binding segment at the membrane distal end that can bridge two liposomes via specific base-pair hybridization. A linker at the membrane proximal end is used to control the separation distance between the liposomes. In the presence of these artificial tethers, SNARE-mediated lipid mixing is significantly accelerated, and the maximum fusion rate is obtained with the linker shorter than 40 nucleotides. As a programmable tool orthogonal to any native proteins, the DNA-lipid tethers can be further applied to regulate other biological processes where capturing and bridging of two membranes are the prerequisites for the subsequent protein function.
 
2008
A caged retinoic acid for one- and two-photon excitation in zebrafish embryos - Neveu, Pierre and Aujard, Isabelle and Benbrahim, Chouaha and Le Saux, Thomas and Allemand, Jean-Francois and Vriz, Sophie and Bensimon, David and Jullien, Ludovic
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 473744-3746 (2008)
 
2007
Two-photon uncaging with the efficient 3,5-dibromo-2,4-dihydroxycinnamic caging group - Gagey, Nathalie and Neveu, Pierre and Jullien, Ludovic
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 462467-2469 (2007)