laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
BIOTECHNOLOGY AND BIOENGINEERING 


1
P U B L I C A T I O N S



 
2007
Propagation of fluorescent viruses in growing plaques - Alvarez, Luis J. and Thomen, Philippe and Makushok, Tatyana and Chatenay, Didicr
BIOTECHNOLOGY AND BIOENGINEERING 96615-621 (2007)

Abstract : To study virus propagation, we have developed a method by which the propagation of the Lambda bacteriophage can be observed and quantified. This is done by creating a fusion protein of the caspid protein gpD and the enhanced yellow fluorescent protein (EYFP). We show that this fusion allows capsid formation and the modified viruses propagate on a surface covered with host bactera thus forming fluorescent plaques. The intensity of fluorescence in a growing plaque determines the distribution of phages. This provides a new tool; to study the propagation of infection at the microscopic level. Bioeng. 2007;96: 615-621. (c) 20066 Wiley Periodicals, Inc.