laboratoire de physique statistique
 
 
laboratoire de physique statistique

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CELLULAR AND MOLECULAR BIOENGINEERING 


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2008
A Nanospring Named Erythrocyte. The Biomembrane Force Probe - Gourier, Christine and Jegou, Antoine and Husson, Julien and Pincet, Frederic
CELLULAR AND MOLECULAR BIOENGINEERING 1263-275 (2008)

Abstract : The Biomembrane Force Probe, BFP, is a sensitive technique that allows the quanti. cation of single molecular bonds. It is a versatile tool that can be used in a wide range of forces (0.1 pN to 1 nN) and loading rates (1-10(6) pN/s). This article describes the principle of the BFP technique, how to set it up and its various advantages. In order to show that this technique is a powerful tool that can be used on a wide range of systems, two different types of applications are presented. The. first example shows how the energy landscape of a single bond can be deduced from the measurements on a well defined pair: the streptavidin-biotin couple. The second example presents a case where cell-cell interactions can be probed at the molecular level: mammalian gametes interactions.
The Surface Force Apparatus to Reveal the Energetics of Biomolecules Assembly. Application to DNA Bases Pairing and SNARE Fusion Proteins Folding - Perez, Eric and Li, Feng and Tareste, David and Pincet, Frederic
CELLULAR AND MOLECULAR BIOENGINEERING 1240-246 (2008)

Abstract : The Surface Force Apparatus (SFA) measures directly, and with nanoscale resolution, the interaction energy vs. distance pro. le of planar arrays of biological molecules (e.g., lipids, polymers, or proteins). Through recent advances in the reconstitution and deposition of lipid bilayers, it is now possible to use SFA to study the interactions between membrane-incorporated biomolecules and to reveal any conformational changes and intermediate assembly states. Therein we describe two example systems. First, we show that using bilayers functionalized to carry DNA bases on their lipid headgroups, we can measure a macroscopic nucleoside nucleoside adhesion force, from which one can obtain a molecular binding energy. Second, we describe the use of the SFA to study the interaction between SNARE proteins, which are involved in most of intracellular fusion events. Membrane fusion occurs when SNARE proteins assemble between lipid bilayers in the form of SNAREpins. SFA measurements between SNAREs embedded in lipid bilayers allowed us to elucidate the energetics and dynamics of SNAREpin folding, and to capture an intermediate binding state in SNAREpin assembly.