laboratoire de physique statistique
 
 
laboratoire de physique statistique

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COLLOIDS AND SURFACES B-BIOINTERFACES 


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P U B L I C A T I O N S



 
2014
Interfacial pressure and phospholipid density at emulsion droplet interface using fluorescence microscopy - Delacotte, Jerome and Gourier, Christine and Pincet, Frederic
COLLOIDS AND SURFACES B-BIOINTERFACES 117545-548 (2014)

Abstract : Phospholipids are widely used to stabilize oil in water micron size emulsion droplets; the interfacial phospholipid density and tension of such droplets are difficult to estimate. In the present paper, we describe a simple approach by which the measurement of a micron size oil droplet interface fluorescence intensity provides directly both the interfacial phospholipid density and the interfacial tension. This method relies on two prior calibration steps: (i) the quantitative variation of the interfacial tension with fluorescence intensity at droplets interface through micro-manipulation techniques; (ii) the variation of interfacial tension with phospholipid density through monolayer isotherm. Here, we show the validity of this approach with the example of micron size oil droplets stabilized with a phosphatidylcholine phospholipid, in aqueous buffer. (C) 2013 Elsevier B.V. All rights reserved.