laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

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FEBS JOURNAL 


1
P U B L I C A T I O N S



 
2011
Nucleosome-remodelling machines and other molecular motors observed at the single-molecule level - Lavelle, Christophe and Praly, Elise and Bensimon, David and Le Cam, Eric and Croquette, Vincent
FEBS JOURNAL 2783596-3607 (2011)

Abstract : Through its capability to transiently pack and unpack our genome, chromatin is a key player in the regulation of gene expression. Single-molecule approaches have recently complemented conventional biochemical and biophysical techniques to decipher the complex mechanisms ruling chromatin dynamics. Micromanipulations with tweezers (magnetic or optical) and imaging with molecular microscopy (electron or atomic force) have indeed provided opportunities to handle and visualize single molecules, and to measure the forces and torques produced by molecular motors, along with their effects on DNA or nucleosomal templates. By giving access to dynamic events that tend to be blurred in traditional biochemical bulk experiments, these techniques provide critical information regarding the mechanisms underlying the regulation of gene activation and deactivation by nucleosome and chromatin structural changes. This minireview describes some single-molecule approaches to the study of ATP-consuming molecular motors acting on DNA, with applications to the case of nucleosome-remodelling machines.