laboratoire de physique statistique
 
 
laboratoire de physique statistique

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2007
Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain - Perier, Aurelie and Gourier, Christine and Pichard, Sylvain and Husson, Julien and Lajeunesse, Evelyne and Babon, Aurelie and Menez, Andre and Gillet, Daniel
FEBS LETTERS 5815480-5484 (2007)

Abstract : We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.