laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
JOURNAL OF IMMUNOLOGY 


2
P U B L I C A T I O N S



 
2016
1
Persisting fetal clonotypes influence the structure and overlap of adult human TCR repertoires - Pogorelyy, M. and Elhanati, Y. and Marcou, Q. and Sycheva, A. and Komech, E. and Britanova, O. and Chudakov, D. and Mamedov, I and Lebedev, Y. and Mora, T. and Walczak, A.
EUROPEAN JOURNAL OF IMMUNOLOGY 4652 (2016)
 
2015
A Reassessment of IgM Memory Subsets in Humans - Bagnara, Davide and Squillario, Margherita and Kipling, David and Mora, Thierry and Walczak, Aleksandra M. and Da Silva, Lucie and Weller, Sandra and Dunn-Walters, Deborah K. and Weill, Jean-Claude and Reynaud, Claude-Agnes
JOURNAL OF IMMUNOLOGY 1953716-3724 (2015)

Abstract : From paired blood and spleen samples from three adult donors, we performed high-throughput VH sequencing of human B cell subsets defined by IgD and CD27 expression: IgD(+)CD27(+) (''marginal zone [MZ]''), IgD(-)CD27(+) (''memory,'' including IgM [''IgM-only''], IgG and IgA) and IgD 2 CD27(-) cells (''double-negative,'' including IgM, IgG, and IgA). A total of 91,294 unique sequences clustered in 42,670 clones, revealing major clonal expansions in each of these subsets. Among these clones, we further analyzed those shared sequences from different subsets or tissues for VH gene mutation, H-CDR3-length, and V-H/J(H) usage, comparing these different characteristics with all sequences from their subset of origin for which these parameters constitute a distinct signature. The IgM-only repertoire profile differed notably from that of MZ B cells by a higher mutation frequency and lower V(H)4 and higher J(H)6 gene usage. Strikingly, IgM sequences from clones shared between the MZ and the memory IgG/IgA compartments showed a mutation and repertoire profile of IgM-only and not of MZ B cells. Similarly, all IgM clonal relationships (among MZ, IgM-only, and double-negative compartments) involved sequences with the characteristics of IgM-only B cells. Finally, clonal relationships between tissues suggested distinct recirculation characteristics between MZ and switched B cells. The ``IgM-only'' subset (including cells with its repertoire signature but higher IgD or lower CD27 expression levels) thus appear as the only subset showing precursor-product relationships with CD27(+) switched memory B cells, indicating that they represent germinal center-derived IgM memory B cells and that IgM memory and MZ B cells constitute two distinct entities.