laboratoire de physique statistique
 
 
laboratoire de physique statistique

Publications

Rechercher
PLOS GENETICS 


3
P U B L I C A T I O N S

S E L E C T I O N N E R
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2014
Six Homeoproteins and a Iinc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype - Sakakibara, Iori and Santolini, Marc and Ferry, Arnaud and Hakim, Vincent and Maire, Pascal
PLOS GENETICS 10 (2014)

Abstract : Thousands of long intergenic non-coding RNAs (lincRNAs) are encoded by the mammalian genome. However, the function of most of these lincRNAs has not been identified in vivo. Here, we demonstrate a role for a novel lincRNA, linc-MYH, in adult fast-type myofiber specialization. Fast myosin heavy chain (MYH) genes and linc-MYH share a common enhancer, located in the fast MYH gene locus and regulated by Six1 homeoproteins. linc-MYH in nuclei of fast-type myofibers prevents slow-type and enhances fast-type gene expression. Functional fast-sarcomeric unit formation is achieved by the coordinate expression of fast MYHs and linc-MYH, under the control of a common Six-bound enhancer.
 
2013
Six Homeoproteins Directly Activate Myod Expression in the Gene Regulatory Networks That Control Early Myogenesis - Relaix, Frederic and Demignon, Josiane and Laclef, Christine and Pujol, Julien and Santolini, Marc and Niro, Claire and Lagha, Mounia and Rocancourt, Didier and Buckingham, Margaret and Maire, Pascal
PLOS GENETICS 9 (2013)

Abstract : In mammals, several genetic pathways have been characterized that govern engagement of multipotent embryonic progenitors into the myogenic program through the control of the key myogenic regulatory gene Myod. Here we demonstrate the involvement of Six homeoproteins. We first targeted into a Pax3 allele a sequence encoding a negative form of Six4 that binds DNA but cannot interact with essential Eya co-factors. The resulting embryos present hypoplasic skeletal muscles and impaired Myod activation in the trunk in the absence of Myf5/Mrf4. At the axial level, we further show that Myod is still expressed in compound Six1/Six4:Pax3 but not in Six1/Six4:Myf5 triple mutant embryos, demonstrating that Six1/4 participates in the Pax3-Myod genetic pathway. Myod expression and head myogenesis is preserved in Six1/Six4:Myf5 triple mutant embryos, illustrating that upstream regulators of Myod in different embryonic territories are distinct. We show that Myod regulatory regions are directly controlled by Six proteins and that, in the absence of Six1 and Six4, Six2 can compensate.
 
2010
Frail Hypotheses in Evolutionary Biology - Ninio, Jacques
PLOS GENETICS 6 (2010)