You can also become a sponsor of this workshop by contacting us.


We also want to thank :The city of TOURS , Merck  for their support.



 

SINGLE MOLECULE BIOPHYSICS


    This EMBO workshop will be held in Tours (Loire valley, France )  between the 8th and 15th  of July 1999. It will gather  about one hundred  physicists, chemists and biologists studying single molecule biophysics. The purpose of this workshop is to gather the specialists in this field together with interested students. Lectures will first describe the different techniques involved in single molecule experiments (optical  and magnetic tweezers,  two-photon excitation, fluorescence correlation  spectroscopy, evanescent wave fluorescence microscopy,  near field scanning probe microscopy, etc..)  and then will focus on more specialized topics (DNA, molecular motors, single enzymatic reactions etc...).

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Invited Speakers     Location and date   Accomodation TravelProgramme Talk's titles Bibliography Short communications proposedWho applied RegistrationPostersand contribured talks Preparing your talk and available materials OrganizersWhy was I rejected ?    Can I keep a contact ?    How can I survive if I don't speak frenh

 

 
 











































Invited  speakers :

A.Adams, P. Barbara, H. Berg,   D. Branton,  C. Bustamante,  H.Gaub,  J. Gelles, Y.Goldman,  F. Heslot, E. Isacoff,  P. Hansma,
R. Hochstrasser,  K. Kinosita, S. Lindsay,  J. Marko, M.Orrit, O.Orwar, J. Prost, S. Quake, R. Rigler, D. Rokhsar,  G. Schenter,
T. Schmidt, P. Selvin,  S. Siegelbaum, F. Sigworth, J. Spudich,  R. Vale, W.W. Webb,  X.S. Xie, T. Yanagida,  E.S. Young.
 

Location and Date :

The workshop will be held from July  8  in the morning to July 15  in the afternoon in a XIX  century Manor at :

Domaine de "la Croix Montoire"
8 bis place Choiseul
37 100 TOURS (FRANCE)
Tel. : 33 -2 47 42 57 68
Fax : 33- 2 47 49 18 32

A nice web site about Tours (in french)

Participants are expected to arrive on July 7 and register at the SMB office in the Manor, starting 2 PM.

Who applied :

    Both young researchers (student and postdocs) and specialists applied to this workshop. The deadline for the registration was April 25th and we were more than twice oversubscribed. The selection of the candidates relied upon the speakers acting as referees of the candidates in their field and evaluating their participation on the basis of their work and letter of intent .

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Accommodation :

    Participants will be housed  in student accommodation  located in the manor i.e. in individual rooms with collective facilities.  Breakfast and lunch will be served  in the manor.  Participants will be free to  have dinner either  in the center of TOURS  (five minute walk) or use the common kitchen.
 

Conference Posters :

    You can help us by posting one of the conference posters in your university or working place :

    A4 or Letter size poster in Acrobat file format (.pdf)

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Participants' short communications and posters :

We invite all participants to explain their work either by giving a short talk (we have reserved 18 talks of 25 minutes in the programme) or to present a poster. In both case, send us, by email before June 15th, an abstract no more than one page using the prepared HTML format abtract form that you can fill in using your prefered browser in composer mode. If this sounds to complicate you can also send us a one page abstact (12 pt, 1.5 interline) either in plain text, in Latex or in RTF format to Vincent.Croquette@physique.ens.fr. We shall inform you if your oral communication is acceped arround June 20th. The list of the abstracts already recieved can be viewed on this page.

Travel :

    Arriving by car, Tours is connected from Paris by the  A10 high way until you see the sign Tours center, you will find here a general map for Tours area,    a general road map,  a city center road map

A detailed Tours web site with various maps (in french)

    Travel arrangements should be made by the participants who are expected to arrive on July 7 and leave on July 15 in the afternoon. Please notice that Tours is located south from Paris. It is within one hour from Paris by fast train (TGV). The name of the TGV train station near Tours is Saint Pierre des Corps. Driving from Paris to Tours is also possible but won't be so fast (3 hours). We recommend that as soon as you receive our agreement for your participation to the conference, you purchase a plane and train ticket for Tours.

    You can find a schematic map of Paris area explaining the access between airports and train station

    To buy a RER ticket at the teller you can use one of these french forms

Paris has two airports : Charles de Gaulle (Roissy) in the north and Orly in the south. Most international flights arrive in Charles de Gaulle (CDG). TGV Trains for Tours depart either from Massy-TGV station (near Orly), CDG-TGV station (inside CDG airport)  or from Montparnasse Station (downtown Paris). On July the 7th, there are five trains starting from CDG-TGV, whereas there are nine starting Massy-TGV station and  fifteen departing from Montparnasse station. We list below the timetable for the three stations. Depending on your schedule you should check the optimal solution taking into account that connection between CDG and Massy takes 1h00 but is direct by RER-B,  CDG to Montparnasse station takes 1h15 but requires taking RER-B and the subway, Orly to Massy-TGV takes 0h20 and is very convenient. Your choice will probably be dictated by your flight's arrival time. If  you can choose the airport  when purchasing your flight tickets, choose Orly.

Trains departing from Paris and arriving in Tours

    Be aware that the TGV station of Tours is located at a few kilometers from  downtown Tours. The name of this TGV station is Saint Pierre des Corps. At this station, you have to leave the TGV and take a normal train which will take you to Tours downtown station. The connection is done for all TGVs and is very quick (5 or 10 minutes). This correspondance is announced (in french) when the TGV arrives, it is also indicated on panels and is quite easy. However if you have to purchase your TGV ticket you might have to indicate that your destination is Saint Pierre des Corps.

When you take the TGV, you must have your ticket punched by a red machine BEFORE you get on the train. Don't forget to do it otherwhise, you will have to pay an extra fee on the train!

To buy a TGV ticket at the teller you can use one of these french forms

From Roissy (95) to Tours on July 7th

Charles de Gaulle TGV station  Saint Pierre des Corps  (connection to Tours)
7.45 am 9.20 am
8.55 am 10.35 am
1.45 pm 3.20 pm
6.54 pm 8.43 pm
9.29 pm 11.06pm

From Massy TGV (91)  to Tours on July 7th

Massy  TGV station  Saint Pierre des Corps  (connection to Tours)
7.02 am 7.54 am
8.31 am 9.20 am
8.56 am 9.47 am
9.46 am 19.35 am
2.31 pm 3.20 pm
3.49 pm 4.39 pm
7.48 pm 8.43 pm
8.47 pm 9.37 pm
1O.14 pm 11.06 pm

From Paris downtown  to Tours on July 7th

Montparnasse  TGV station  Saint Pierre des Corps  (connection to Tours)
6.50 am 7.54 am
7.40 am 8.43 am
9.05 am 10.01 am
10.45 am 11.41 am
12.35 am 13.27 am
2.05 pm 3.01 pm
3.25 pm 4.23 pm
4.35 pm 5.31 pm
5.15 pm 6.11 pm
5.40 pm 6.36 pm
6.05 pm 7.09 pm
6.40 pm 7.42 pm
6.50 pm 7.46 pm
7.35 pm 8.38 pm
10.15 pm 11.12 pm

To buy a RER ticket at the teller you can use one of these french forms

    From Orly to Massy-TGV station, the fastest and easiest connection is to take the ORLYVAL to Antony (RER-B station and terminus of OrlyVal) and then take the RER-B southwards (opposite to Paris direction) heading to St Rémy-les-Chevreuses or Orsay or Massy-Palaiseau.  Buy your ticket in Orly directly to Massy-Palaiseau (approx 50FF or 8$). All RER-B trains stop in Antony and in Massy-Palaiseau where you should leave the RER-B train.  The Massy-TGV station and the Massy-Palaiseau RER-B station are very close (200 meters). Look for the sign MASSY-TGV.

    From Charles de Gaulle (Roissy)  to Massy-TGV station, After purchasing a ticket to Massy-Palaiseau (approx 50FF), take the RER-B train directly inside Roissy airport, check that the train actually stops in Massy-Palaiseau (the panel on the platform must mention Massy-Palaiseau). Get out of the RER-B train in Massy-Palaiseau. The Massy-TGV station and the Massy-Palaiseau RER-B station are very close (200 meters). Look for the sign MASSY-TGV.

    From Charles de Gaulle (Roissy)  to Montparnasse TGV station : buy a RER ticket for Paris downtown, it will cover both RER and subway trip. Take the RER-B and stop at the station named Denfert-Rochereau. Follow the orange sign indicating "correspondance" to acces directly to the subway. You should look for the "Nation/Etoile" line 6, take the subway in the direction of Etoile. After a few stops, you should reach "Montparnasse Bienvenue" subway station where you leave the subway. Look then for the sign indicating Montparnasse SNCF train station. After some corridors you should arrive to the TGV station.
 
 

Paris subway map Paris subway and RER map    Paris RER map   Paris ground transportation agency (RATP)














You should make your TGV reservation in advance (You can purchase your ticket with your credit card by calling 33 -836 35 35 39, an english speaking operator should answer you. You should then be able to withdraw your ticket in the train station on teller just by giving a code number provided by the operator). Your final destination is "Tours centre ville" (Tours center). You will be able to get the corresponding ticket in any Railway station (SNCF) in particular in Massy-TGV.

    Arriving in Tours , the workshop location (Domaine de la Croix-Montoire) can be joined by bus from both Tours railway stations:
- Saint-Pierre des Corps: the TGV railway station, (nearly all TGV have a corresponding train connecting them to Tours central Station)
- Tours centre. (central rail station)
    From Saint-Pierre des Corps, take bus  line 3 and change at Place Jean Jaurès. Then take bus  lines 1, 2, 8, 9 or 13 northwards to the avenue de la Tranchée. Once the Loire river is crossed, get out. The gate to the Domaine de la Croix-Montoire is nearby. (Tours city map, downtown map) To bget a french taxi  you can use this french forms
    From Tours centre,  take  bus  lines 1, 2, 8, 9 or 13 northwards to the avenue de la Tranchée. Once the Loire river is crossed, get out. The gate to the Domaine de la Croix-Montoire is nearby.

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Registration :

    The registration fees is 2000 FF for french participants and  $400 for others; grants are available.
    If you have received a message explaining that we accept your participation to the workshop, you should pay your registration by sending us a Fax with your name, the type (VISA, MC) and number of your credit card, the expiration date and a sentence saying that you accept that we debit your account of the corresponding amount.
ed to Vincent.Croquette@physique.ens.fr.

The Deadline for accepted participants to confirm their participation by paying their registration is May 30th, 1999. After this date, we shall propose unconfirmed position to those on the waiting list.
 
 

NAME AND ADDRESS OF ORGANIZERS

 David Bensimon 1, Vincent Croquette,1 Ludovic Jullien 2and  Shimon Weiss,3

    Ecole Normale Supérieure, Laboratoire de Physique Statistique 24 rue Lhomond, Paris 75005
So far registration is closed.
  • However if you are thinking that this conference is a tremendous opportunity considering your present or future research activity, you can still send us a preregistration, we will add it to the waiting list (which is already quite big). A preregistration form is available and  can be mailDavid.Bensimon@physique.ens.fr , Vincent.Croquette@physique.ens.fr   Tel (33 )1  44 32 34 96 Fax  (33)1 44 32 34 33
    1. Ecole Normale Supérieure,  Departement de Chimie, 24 rue Lhomond, Paris 75005 jullien@junie.ens.fr   Tel (33 )1  44 32 33 33 Fax ( 33) 1 44 32
    2. Laurence Berkeley Natnl. Lab., MS 2-300,  1 Cyclotron road, Berkeley, CA 94720 , sweiss@mh1.lbl.gov, Tel.  ( 1)  510 486 52 02  Fax. (1) 510 486 55 30
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    Tentative programme

        You will find here the main lines of the workshop, the precise timing of each speaker is not yet fixed, but the major events are described. You will find in the next array the title of each speaker. Changes are still possible. We are planning to have 18 talks of 30 min. to be given by the participants. Please send us a title and an abstract for your possible talk.


    If you want to print the programme, use this acrobat file :smallprog.pdf

    Thursday 8th
    8.30 am to 1 pm Micromanipulation techniques: Optical /Magnetic tweezers, AFM  S. Quake, V. Croquette,  P. Hansma, S. Lindsay
    2.30 pm to 5.30 pm Guided visit of  old Tours 
    6 pm to 7 pm Single molecule fluorescence F. Keilman, C.A.M. Seidel
    7.30 pm to 9 pm Cocktail at the city Hall of Tours
    Friday 9th
    8.30 am to 1 pm Single molecule  fluorescence/ ionic channels S. Weiss, M. Orrit, E.Isacoff, F.Sigworth
    2pm to 5 pm Practical workshop on micromanipulation Posters , demos and discussions
    5 pm to 7.30 pm ionic channels P.Selvin, S.Sieglelbaum,A.Pralle
    Saturday 10th  
    8.30 am to 1 pm Single molecule at work Ligand / receptor interaction enzymatic activity H.Gaub, S.Xie, E.Yeung, Wennalm S., G. Bonnet
    2 pm to 5  pm Posters, demos and discussions
    5 pm to 7.30 pm Protein folding and structure R.Hochstrasser, D.Rokhsar,W.A. Eaton
    Sunday 11th
    8.30 to 1pm Protein folding and structure/ Photo-physics on single molecules T.Schmidt, P.Barbara, S.Adams, G.Schenter
    2 pm to 5 pm  Posters, demos and discussions
    5 pm to 7.30 pm Protein folding and structure/ Photo-physics on single molecules Y.Goldman,J.Hofkens, X.Zhuang, G.Zocchi
    Monday 12th
    8.30 am to 1 pm Molecular motors H.Berg, K.Kinoshita, J.Spudich,L.Berger, A. Ishijima
    2 pm to 4.pm Posters, demos and discussions
    4 pm to 6 pm Molecular motors T.Yanagida, R.Vale
    6.30 pm to 11 pm Visit of Montlouis cellar and dinner
    Tuesday 13th
    8.30 am to 1 pm Single molecule technologies O.Orwar, S.Allen, M.Auer, M.Sauer, V. Uhl., D.Branton
    2 pm to 10  pm Social event:  visit of Chenonceaux  and Amboise
    10  pm Tours by night
    Wednesday 14th
    8.30 am to 1 pm Theory of molecular motors DNA structures J.Prost, J. Marko, F.Heslot, R.Lavery, 
    2 pm to 5 pm Posters, demos and discussions
    5 pm to 7.30 pm DNA /Prospectives S.B.Smith,  round table
    8pm to next morning Celebration of Bastille day Firework and dancing
    Thursday 15th
    8.30 am to 1. pm DNA/protein interactions  C.Bustamante, J.Gelles, T.Ha, B.Maier, T.Strick,D.Keller
    12.30 pm to 2 pm  Lunch
    afternoon departure

    Detailed Programme
    If you want to print the programme, use this acrobat file :programme.pdf

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    8th

    Morning session  Micromanipulation techniques:  Optical tweezers Magnetic tweezers, AFM micromanipulation
    8.30 am to 9.30 am QUAKE Steven Imaging information in DNA- from base pairs to genomic dynamics
    9.30 am to 10.30 am CROQUETTE Vincent Magnetic tweezers and DNA supercoiling
    coffe break
    11.00 am to 12.00 am HANSMA Paul Single-Molecule science with scanning probes
    12.00 am to 1.00 pm LINDSAY Stuart Dynamic force microscope for imaging and manipulating single molecules at the liquid-solid interface
    1.00 pm to 2.00 pm lunch
    2.30 pm to 5.30 pm Visit of old Tours  we shall cross the Loire river and  have a guided tour of  the old city.
    Afternoon session:Single molecule fluorescence
    6.00 pm to 6.30 pm KEILMANN Fritz  Vibrational-infrared Near-Field Microscopy
    6.30 pm to 7.00 pm SEIDEL C. A. M.,  Multi-dimensional State-selective Fluorescence Spectroscopy  of Single DNA-Molecules in Solution
    7.15 pm to 7.30 pm Walk from la Croix Montoire to City Hall
    7.30 pm to 9.00 pm Cocktail at the City Hall of Tours 
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    9th

    Morning session:    Single molecule fluorescence/Ionic channels
    8.30 am to 9.30 am WEISS Shimon Observing Single Molecule reactions
    9.30 am to 10.30 am ORRIT Michel Optical detection and study of Single-Molecules
    coffee break
    11.00 am to 12.00 am ISACOFF Ehud Optical measurement of ion channel gating rearrangements
    12.00 am to 1.00 pm SIGWORTH Fred Intermediate steps in the activation of voltage gated potassium channels
    1.00 pm to 2.00 pm lunch
    2.00 pm to 5.00 pm Posters, demos and discussions
    Afternoon session: Ionic channels
    5.00 pm to 6.00 pm SELVIN Paul Voltage-induced movement in a potassium ion channel: detection by ensemble energy transfer and the potential of single-molecule
    6.00 pm to 7.00 pm SIEGELBAUM Steven Contribution of individual ligand binding events to the activation of cyclic nucleotid-gated channels
    7.00 pm to 7.30 pm PRALLE, Arnd Local environment of single membrane proteins studied by 3D-thermal noise analysis
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    10th

    Morning session: Single molecule at work: ligand / receptor interaction, enzymatic activity
    8.30 am to 9.30 am GAUB Herman  Probing chemical bonds by Single-Molecule force spectroscopy
    9.30 am to 10.30 am XIE X. Sunney Single-Molecule enzymology
    coffee break
    11.00 am to 12.00 am YEUNG Edward Characterization of Single-Molecules in microenvironments
    12.00 am to 12.30 am WENNMALM Stefan The fluctuating enzyme : a  Single Molecule lApproach
    12.30 am to 1.00 pm BONNET Grégoire Study of DNA conformationnal fluctuations by fluorescence correlation spectroscopy
    1.00 pm to 2.00 pm lunch
    2.00 pm to 5.00 pm Posters, demos and discussions
    Afternoon session: Protein Folding and structure.
    5.00 pm to 6.00 pm HOCHSTRASSER Robin  Folding of GCN-4-fs on silica and structures of single LH2 complexes
    6.00 pm to 7.00 pm ROKHSAR Daniel Transition states and intermediates in Single-Molecule models for protein folding
    7.00 pm to 7.30 pm EATON William A. Kinetics and Mechanisms in Protein Folding
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    11th

    Morning session: Protein folding and structure/Photo-physics on single molecules
    8.30 am to 9.30 am SCHMIDT Thomas Dynamical behaviour of membrane components
    9.30 am to 10.30 am BARBARA Paul Energy funnels, folded conformations and energy transfer domains in Single conjugated polymer molecules
    coffee break
    11.00 am to 12.00 am ADAMS Stephen Targeting of fluorescent indicators in living cells
    12.00 am to 1.00 pm SCHENTER Greg Statistical analysis of Single-Molecule enzymatic dynamics
    1.00 pm to 2.00 pm lunch
    2.00 pm to 5.00 pm Posters, demos and discussions
    Afternoon session: Protein folding and structure/Photo-physics on single molecules
    5.00 pm to 6.00 pm GOLDMAN Yale Single-Molecule structural dynamics by fluorescence polarization microscopy
    6.00 pm to 6.30 pm HOFKENS J. Spectroscopy and microscopy of a single multichromophoric dendrimer macromolecule.
    6.30 pm to 7.00 pm ZHUANG Xiaowei Folding/unfolding of single protein and RNA molecules observed by fluorescence
    7.00 pm to 7.30 pm ZOCCHI Giovanni  Plastic deformation of a protein 
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    12th

    Morning session: Molecular motors
    8.30 am to 9.30 am BERG Howard  Physiology of the flagellar rotary motor
    9.30 am to 10.30 am KINOSITA Kazuhiko Huge and small tags for Single-Molecule physiology
    coffee break
    11.00 am to 12.00 am SPUDICH Jim Single-Molecule biomechanics : the myosin family of molecular motors
    12.00 am to 12.30 am BERGER L.  Structural dynamics of myosin detected by single molecule fluorescence polarization.
    12.30 am to 1.00 pm ISHIJIMA Akihiko  Simultaneous Measurement of Individual ATPase and Mechanical Reactions by a Single Myosin  Molecule at Work
    1.00 pm to 2.00 pm lunch
    2.00 pm to 4.00 pm Posters, demos and discussions
    Afternoon session:Molecular motors
     4.00 pm to  5.00 pm YANAGIDA Toshio Single -Molecule imaging and nanomanipulation of molecular motors at work
    5.00 pm to 6.00 pm VALE Ronald  Tracking single molecule kinesin motility by fluorescence microscopy 
    6.30 pm to 11.00 pm Bus ride to visit  Montlouis cellar and dinner
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    13th

    Morning session:  Single molecule technologies
    8.30 am to 9.30 am ORWAR Owe Biochemical reaction dynamics in ultra small biomimetic containers
    9.30 am to 10.00 am ALLEN S. Measuments of biomolecular interactions in pharmaceutical and immunodiagnostic
    10.00 am to 10.30 am AUER Manfred  Interfacing Novel Detection Technologies in Single Molecule Spectroscopy with Drug Discovery.
    coffee break
    11.00 am to 11.30 am SAUER M.  Detection, identification and handling of single biomolecules using semiconductor lasers and microelectrophoresis.
    11.30 am to 12.00 am UHL, Volker From single molecule biophysics to single molecule biochemistry: Observation of reactions in the light microscope.
    12.00 am to 1.00 pm BRANTON Daniel Prospects for nanopore sequencing 
    1.00 pm to 2.00 pm lunch
    2.00 pm to 10.00 pm Social event:  visit of Chenonceaux  and Amboise
    10.00 pm to ... Tours by night
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    14th

    Morning session: Theory of molecular motors / DNA
    8.30 am to 9.30 am PROST Jacques From single to many molecular motors
    9.30 am to 10.30 am MARKO John Structure, Viscoelasticity and Plasticity of Mitotic Chromosomes
    coffee break
    11.00 am to 12.00 am HESLOT François Time resolved force measurement on the opening and closing of DNA
    12.00 am to 1.00 pm LAVERY Richard Modeling studies linking DNA mechanics, dynamics and recognition
    1.00 pm to 2.00 pm lunch
    2.00 pm to 5.00 pm Posters, demos and discussions
    Afternoon session:        DNA/ prospectives
    5.00 pm to 5.30 pm SMITH Steven B.  Polymerization and Mechanical Properties of Single RecA-DNA Filaments
    5.30 pm to 7.30 pm Round table Prospectives 
    8pm to next morning Celebration of Bastille day. Firework and dansing
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    15th

    Morning session:        DNA/protein interactions
    8.30 am to 9.30 am BUSTAMANTE Carlos Following transcription one molecule at a time
    9.30 am to 10.30 am GELLES Jeff Studying motor enzymes one molecule at a time
    coffe break
    11.00 am to 11.30 am T. Ha Kinetics of Single DNA unwinding by Rep Helicase
    11.30 am to 12.00 am David Keller Mechanochemical Models for Molecular Motors
    12.00 am to 12.30 am B.Maier Activity of DNA polymerase observed by elastic measurements on single DNA molecules
    12.30 am to 13.00 am T. Strick Single-molecule analysis of TopoisomeraseII/DNA interactions
    12.30 Lunch and departure

     
     

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     Talk's titles

     
    E
       
    INVITED SPEAKERS
    Talk's TITLE 
    M
     
       
    ADAMS Stephen
    « Targeting of fluorescent indicators in living cells »
    B
     
       
    BARBARA Paul
    « Energy funnels, folded conformations and energy transfer domains in Single onjugated polymer molecules ».
    O
     
       
    BERG Howard 
    « Physiology of the flagellar rotary motor »
       
       
    BRANTON Daniel
    « Prospects for nanopore sequencing »
    W
     
       
    BUSTAMANTE Carlos
    « Following transcription one molecule at a time »
    O
     
       
    CROQUETTE Vincent
    « Magnetic tweezers and DNA supercoiling »
    R
     
       
    GAUB Herman 
    « Probing chemical bonds by Single-Molecule force spectroscopy »
    K
     
       
    GELLES Jeff
    « Studying motor enzymes one molecule at a time » 
    S
     
       
    GOLDMAN Yale
    « Single-Molecule structural dynamics by fluorescence polarization microscopy »
    H
     
       
    HANSMA Paul
    « Single-Molecule science with scanning probes »
    O
     
       
    HESLOT François
    « Time resolved force measurement on the opening and closing of DNA »
    P
     
       
    HOCHSTRASSER Robin 
    « Folding of GCN-4-fs on silica and structures of single LH2 complexes »
    on
     
       
    ISACOFF Ehud
    « Optical measurement of ion channel gating rearrangements »
    S
     
       
    KINOSITA Kazuhiko
    « Huge and small tags for Single-Molecule physiology »
    I
     
       
    LAVERY Richard
    « Modeling studies linking DNA mechanics, dynamics and recognition »
    N
     
       
    LINDSAY Stuart
    « Dynamic force microscope for imaging and manipulating single molecules at the liquid-solid interface »
    G
     
       
    MARKO John
    « Structure, Viscoelasticity and Plasticity of Mitotic Chromosomes »
    L
     
       
    ORRIT Michel
    « Optical detection and study of Single-Molecules »
    E
     
       
    ORWAR Owe
    « Biochemical reaction dynamics in ultra small biomimetic containers »
       
       
    PROST Jacques
    « From single to many molecular motors »
    M
     
       
    QUAKE Steven
    « Imaging information in DNA- from base pairs to genomic dynamics »
    O
     
       
    RIGLER Rudolph
    « Enzyme catalysis at the Single Molecule level »
    L
     
       
    ROKHSAR Daniel
    « Transition states and intermediates in Single-Molecule models for protein folding »
    E
     
       
    SCHENTER Greg
    « Statistical analysis of Single-Molecule enzymatic dynamics »
    C
     
       
    SCHMIDT Thomas
    « Dynamical behaviour of membrane components »
    U
     
       
    SELVIN Paul
    « Voltage-induced movement in a potassium ion channel: detection by ensemble energy transfer and the potential of single-molecule » 
    L
     
       
    SIEGELBAUM Steven 
    « Contribution of individual ligand binding events to the activation of cyclic nucleotid-gated channels » (leaves 13-14)
    E
     
       
    SIGWORTH Fred
    « Intermediate steps in the activation of voltage gated potassium channels » (leaves 14)
       
       
    SPUDICH Jim 
    « Single-Molecule biomechanics : the myosin family of molecular motors »
    B
     
       
    VALE Ronald 
    « Tracking single molecule kinesin motility by fluorescence microscopy »
    I
     
       
    WEBB Watt 
    « Fast broad band dynamics for Single-Molecules »
    O
     
       
    WEISS Shimon
    « Observing Single Molecule reactions »
    P
     
       
    XIE X. Sunney
    « Single-Molecule enzymology »
    H
     
       
    YANAGIDA Toshio
    « Single -Molecule imaging and nanomanipulation of molecular motors at work »
    Y
     
       
    YEUNG Edward 
    « Characterization of Single-Molecules in microenvironments »
    S
     
       
       
    I
     
       
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    C
     
       
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    S
     
       
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    Bibliography

        To prepare this workshop, you can already improve your knowledge by reading some (or all) the articles listed below. These articles have been given by the speakers in order to support their talk. They are available in Acrobat format (*.pdf) by clicking on their title.(This part is not yet finished !)
     
     
    Pr ADAMS Stephen
    Dept. of Pharmacology
    University of California
    SAN DIEGO, CA 92093-0647
    USA
    e-mail : sadams@ucsd.edu
    Dynamic and quantitative Ca2+ measurements using improved cameleons
    A. Miyawaki, O Griesbeck, R. Heim and R.Y. Tsien
    PNAS 96 pp. 2135-2140, 1999
    Specific Covalent Labeling of Recombinant Protein Molecules inside Live Cells
    B. A. Griffin, S.R. Adams, R.Y. Tsien
    Science 281, pp.  269-272, 1998
    Pr  BARBARA Paul
    Department of Chemistry and Biochemistry
    The University of Texas, Welch Hall
    AUSTIN, TX 78712
    USA
    e-mail : p.barbara@mail.utexas.edu
    Pr  BERG Howard C.
    Dept. of Molecular & Cellular Biology
    Harvard University
    16 Divinity Avenue 
    CAMBRIDGE, MA 02138
    USA
    e-mail : hberg@biosun.harvard.edu
    Symmetries in bacterial motility
    Howard C. Berg
    PNAS 93 , pp. 14225-14228, 1996
    Control of direction of flagellar rotation in bacterial chemotaxis
    Birgit E. Scharf, Karen A. Fahrner, Linda Turner and Howard C. Berg
    PNAS 95, pp. 201-206, (1998)
    Absence of a barrier to backwards rotation of the bacterial flagellar motor demonstrated with optical tweezers
    Richard M. Berry and Howard C. Berg
    PNAS 94, pp. 14433-14437, (1997)
    Pr  BRANTON Daniel
    Dept. of Molecular & Cellular Biology
    Harvard University
    16 Divinity Avenue 
    CAMBRIDGE, MA 02138
    USA
    e-mail : dbranton@harvard.edu
    Characterization of individual polynucleotide molecules using a membrane channel
    John J. Kasianowicz, Eric Brandin, Daniel Branton, and David W. Deamer
    PNAS 93, pp. 13770-13773, (1996)
    Pr  BUSTAMANTE Carlos
    Department of Molecular and Cell Biology
    University of California
    229 Stanley Hall # 3206
    Berkeley, CA 94720-3206
    USA
    e-mail : carlos@alice.berkeley.edu
    Overstreching B-DNA : The elastic response of individual double-stranded and single-stranded DNA molecules
    Steven B. Smith, Yujia Cui and Carlos Bustamante
    Science 271, pp. 795-798, (1996)
    Folding-unfolding transitions in single titin molecules characterized with lazers tweezers
    Science 276, 1112-1116, (1997)
    Pr  CROQUETTE Vincent
    Laboratoire de Physique Statistique
    Ecole Normale Supérieure 
    24, rue Lhomond
    75005 PARIS
    FRANCE
    e-mail : Vincent.Croquette@physique.ens.fr
    T. Strick, J.F. Allemand, D. Bensimon, A. Bensimon, and V.Croquette. The elasticity of a single supercoiled DNA molecule. Science, 271: 1835 --1837, 1996. 
    T. Strick, J.-F. Allemand, D. Bensimon, and V. Croquette. The behavior of supercoiled DNA. Biophysical Journal, 74: 2016--2028, 1998.
     T. Strick, V. Croquette, and D. Bensimon. Homologous pairing in stretched supercoiled DNA. Proc. Nat. Acad. Sci. (USA), 95: 10579--10583, 1998.
     J.-F. Allemand, D. Bensimon, R. Lavery, and V. Croquette. Stretched and overwound DNA form a Pauling-like structure with exposed bases. Proc. Natl. Acad. Sci. USA, 95, (1998), 14152.
    Pr  GAUB Herman E.
    Angewandte Physik
    München Universität
    Amalienstrasse 54
    80799 MÜNCHEN
    GERMANY
    e-mail : gaub@physik.uni-muenchen.de
    Single molecule force spectroscopy by AFM indicates helical structure of poly(ethylene­glycol) in water 
    F Oesterhelt, M Rief and H E Gaub New Journal of Physics 1 (1999) 6.1--6.11 
    Sequence¡dependent mechanics of single DNA molecules 
    Matthias Rief , Hauke Clausen Schaumann  and Hermann E. Gaub 
    Nature structural biology . volume 6 number 4 . april 1999 p 346
    Elastically Coupled Two­Level Systems as a Model for Biopolymer Extensibility 
    Matthias Rief,  Julio M. Fernandez,  and Hermann E. Gaub 
    Phys. Rev. Lett.81, 21 (1998) p 4754-4757
    Pr  GELLES Jeff
    Dept. of Biochemistry,  MS009
    Brandeis University
    WALTHAM, MA 02454-9110
    USA
    e-mail : gelles@brandeis.edu
    Force and Velocity Measured for Single Molecules of RNA Polymerase; M.D. Wang, M.J. Schnitzer, H. Yin, R. Landick, J. Gelles, S.M. Block. Science 282, (1998), pp 902--907
    Transcription Against an Applied Force; H.Yin, M.D. Wang, K. Svoboda, R. Landick, S. M. Block and J. Gelles; Science270, (1995), pp 1653-1657
    Pr  GOLDMAN Yale
    Dept de Physiology / PMI
    School of Medicine
    University of Pennsylvania
    PHILADELPHIA,  PA 19104-6083
    USA
    e-mail : goldmany@mail.med.upenn.edu
    Model-Independent Analysis of the observation of Fluorescent Probes with Restricted Mobility in Muscle Fibers
    R.E. Dale, S.C. Hopkins, A.A an der Heide, T. Marszalek, M. Irving and Y.E. Goldman; Biophysical Journal 78 1606-1618; (1999).
    Fluorescence Polarization Transients from Rhodamine Isomers on the Myosin Regulatory Light Chain in Skeletal Muscle Fibers
    S.C. Hopkins, C.Sabido-David, J.E.T. Corrie, M. Irving and Y.E. Goldman; Biophysical Journal 74; 3093-me3110; (1998).
    Pr  HANSMA Paul
    Physics Departement
    University of California
    SANTA BARBARA, CA 93106-9530
    USA
    e-mail : prasant@physics.ucsb.edu
    Escherichia Coli  RNA Polymerase activity observed using Atomic Force Microscopy
    Sandor Kasas, Nell H.Thompson, Bettie L. Smith, Helen G. Hansma, Xingshu  Zhu, Martin Guthold, Carlos Bustamante, Eric T. Kool, Mikhali Kashlev, and Paul Hansma
    Biochemistry, vol 36, number 3, pp. 461-468 (1997)
    Pr  HESLOT François
    Laboratoire de Physique de la Matière Condensée
    Ecole Normale Supérieure 
    24, rue Lhomond
    75005 PARIS
    FRANCE
    e-mail : Francois.Heslot@physique.ens.fr
     B. Essevaz-Roulet, U. Bockelmann, and F. Heslot ; Mechanical separation of the complementary strands of DNA  PNAS 1997 94: 11935-11940.
    Molecular Stick-Slip Motion Revealed by Opening DNA with Piconewton Forces  U. Bockelmann, B. Essevaz-Roulet, and F. Heslot Physical Review Letters (1997) 79, pp. 4489-4492
    DNA strand separation studied by single molecule force measurements; U. Bockelmann, B. Essevaz-Roulet, and F. Heslot Physical Review E (1998 ) 58, pp. 2386-2394
    Pr  HOCHSTRASSER Robin M.
    Dept. of Chemistry
    University of Pennsylvania
    PHILADELPHIA, PA 19104
    USA 
      e-mail : hochstra@sas.upenn.edu
    Folding Dynamics of Single GCN­4 Peptides by Fluorescence Resonant 
    Energy Transfer Confocal Microscopy 
    Yiwei Jia, David S. Talaga, Wai Leung Lau, Helen S. M. Lu, William F. DeGrado, Robin M. Hochstrasser (Preprint) 
    Fluorescence and photobleaching dynamics of single light­harvesting complexes 
    MARTIN A. BOPP, YIWEI JIA, LIANGQUAN LI, RICHARD J. COGDELL AND ROBIN M. HOCHSTRASSER 
    Proc. Natl. Acad. Sci. USA Vol. 94, pp. 10630--10635, September 1997
    Nonexponential kinetics of a single tRNA Phe molecule under physiological conditions 
    YIWEI JIA,ALEXANDER SYTNIK,LIANGQUAN LI,SERGUEI VLADIMIROV,BARRY S. COOPERMAN, 
    AND ROBIN M. HOCHSTRASSER 
    Proc. Natl. Acad. Sci. USA Vol. 94, pp. 7932--7936, July 1997 

    Single­molecule spectroscopy with 27 fs pulses: Time­resolved experiments 
    and direct imaging of orientational distributions 
    M. A. Bopp, Y. Jia, G. Haran, E. A. Morlino, and R. M. Hochstrasser a) 
    APPLIED PHYSICS LETTERS  73, NUMBER 1 (1998) p7 

    Pr  ISACOFF Ehud
    Dept. of Molecular and Cell Biology, 271 LSA
    University of California
    BERKELEY, CA 94720-3200
    USA
       e-mail : ehud@uclink.berkeley.edu
    Direct Physical Measurement of Conformational Rearrangement Underlying Potassium Channel Gating. L. M. Mannuzzu, M.M. Moronne and E.Y. Isacoff, Science 271, (1996), pp 213-216
    Protein Rearrangements Underlying Slow Inactivation of the Shaker K- Channel; E. Loots and E.Y. Isacoff; J. Gen. Physiol. 112, (1998) pp 377-389
    A Genetically Encoded Optical Probe of Membrane Voltage; M. S. Siegel and E.Y. Isacoff; Neuron, 19, (1997), pp 735-741.
    Pr KINOSITA Kazuhiko
    Dept. of Physics,
    Keio University
    Hiyoshi 3-14-1, Kohoku-hu
    YOKOHAMA  223-8522
    JAPAN
    e-mail : kazuhiko@phys.keio.ac.jp
    F1-ATPase is a Highly Efficient Molecular Motor that Rotates with Discrete 120° Steps
    R. Yasuda, H. Noji, K. Kinosita, Jr and M. Yoshida; Cell, 93 p. 1117-1124; (1998).
    Axial rotation of sliding actin filaments revealed by single-fluorophore imaging
    I. Sae, H. Miyata, S. Ishiwata and K. Kinosita, Jr; PNAS, 94, p. 5646-5650, (1997).
    Tying a molecular knot with optical tweezers
    Y. Arai, R. Yasuda, K. Akashi, Y. Harada, H. Miyata, K. Kinosita Jr and H. Itoh. Nature in press.
    Pr  LAVERY Richard
    IBPC, Lab. de Biochimie Théorique
    13 rue Pierre et Marie Curie
    75005 PARIS
    FRANCE
    e-mail : richard@ibpc.fr
    Local DNA stretching mimics the distortion caused by the TATA 
    box­binding protein 
    ANNE LEBRUN, ZIPPORA SHAKKED  , AND RICHARD LAVERY 
    Proc. Natl. Acad. Sci. USA Vol. 94, pp. 2993--2998, April 1997 
    A model for parallel triple Helix Formation in RecA: Single Strand Association with a Homologous Duplex via Minor Groove. G. Bertucat, R.Lavery and C. Prévost.Journal of Biomolecular Structure & Dynamics 16 (1998) 535-546
    Modeling the Mechanics of a DNA Oligomer; A. Lebrun and R. Lavery Journal of Biomolecular Structure & Dynamics16 (1998) 593-604
    Pr  LINDSAY Stuart
    Dept. of Physics and Astronomy
    Arizona State University
    P.O.Box 87 1504
    TEMPE, AZ 85287-1504
    USA
    e-mail : Stuart.Lindsay@asu.edu
    Carotene as a Molecular Wire: Conducting Atomic Force Microscopy
    G. Leatherman, E.N. Durantini, D.Gust, T.A. Moore, A.L. Moore, S. Stone, Z. Zhou, P. Rez, Y.Z. Liu and S.M. Lindsay, J. Phys. Chem. B (1998)

    Dynamic Force Microscopy in Fluid
    M. Lautz, Y.Z. Liu, X.D Cui, H. Tokumoto and S.M. Lindsay
    To appear in Surf. Interface Anal. 27, (1999)

    Pr  MARKO John
    Dept of Physics,  MC 273
    University of Illinois 
    CHICAGO, IL 60607-7059
    USA
    e-mail : marko@isolda.phy.uic.edu
    Polymer models of meiotic and mitotic chromosomes
    John F. Marko and Eric D. Siggia
    Molecular Biology of the Cell, 8, pp. 2217-2231 (1997)

    Elasticity and structure of eukaryote chromosomes studied by micromanipulation and micropipette aspiration
    Bahram Houchmandzadeh, John Marko, Didier Chatenay and Albert Libchaber  Biology, 139, pp. 1-12 (1997)

    Reversible and irreversible responses to force by mitotic chromosomes
    Sertac Eroglu, Michael Poirier, Didier Chatenay, and John F. Marko
    Preprint (1999)

    Rec-A binding to a single double strand DNA molecule : A possible role of DNA conformational fluctuations
    J-F. Léger, J. Robert, L. Bourdieu, D. Chatenay and J.F. Marko; PNAS 95 (1998) 12295-12299

    Pr  ORRIT Michel
    CPMOH, Université de Bordeaux I
    351, cours de la Libération
    33405 BORDEAUX
    FRANCE
    e-mail : orrit@yak.cpmoh.u-bordeaux.fr
    Optical spectroscopy of single molecules in solids, M. Orrit et al, in Progress in Optics, 35, Elsevier (1996) (article de revue en profondeur, sur mol. uniques à basse température, un peu ancien maintenant) Optical detection and spectroscopy of single molecules, R. Brown, B. Lounis, and M. Orrit, Europhysics News September/December 1997, p. 174. (revue brève de notre activité) Driving the Bloch vector of a single molecule: towards a triggered single photon source, Ch. Brunel et al., C. R. Acad. Sci. Paris 326 (1998) 911 (principe d'une source de photons uniques et premiers résultats expérimentaux) Illuminating single molecules in condensed matter, W. E. Moerner and M. Orrit, Science 283 (1999) 1670 (revue du domaine 'optique sur mol. uniques', incluant haute et basse température). 
    Pr  ORWAR Owe
    Dept. of Chemistry
    Göteborg University
    Kaemivagen 10
    41296 GÖTEBORG 
    SWEDEN
    e-mail : orwar@amc.chalmers.se
    D. T. Chiu et al, Chemical transformations in individual ultrasmall biomimetic containers. SCIENCE, (1999) 283, 1892- 
    D.T. Chiu et al., Manipulating the nanoenvironment around single molecules contained within vesicles. Chem. Phys. In Press 
    J. A. Lundqvist et al., Altering the biochemical state of individual cells and organelles with ultramicroelectrodes. Proc. Natl. Acad. Sci. USA. (1998) 95, 10356- 
    A. Karlsson et al., A multimicrochannel structure for liposome-selection, transport, and fusion JACS, submitted
    Pr  PROST Jacques
    PCC, Section Recheche
    Institut Curie
    11 rue Pierre et Marie Curie
    75231 PARIS Cédex 05
    France
    e-mail : Jacques.Prost@curie.fr
    Energy Transduction of Isothermal Ratchets: Generic Aspects and Specific Examples Close to and Far from Equilibrium 
    Andrea Parmeggiani, Frank J¨ulicher, Armand Ajdari Lambda and Jacques Prost (preprint)
    Self­organized Beating and Swimming of Internally Driven Filaments 
    S'ebastien Camalet, Frank J¨ulicher and Jacques Prost (preprint)
    Pr  QUAKE Steven
    Dept. of Applied Physics
    Caltech - MS 128-95
    PASADENA, CA 91125
    USA
    e-mail : quake@its.caltech.edu
    .W. Hatfield and S.R. Quake, "The Dynamic Properties of an Extended Polymer", Phys Rev Lett 82: 3548-51 (1999).
     G. Lessard, T.J. Yang, P. Barritault and S. Quake, "A Scanning
     Apertureless Fluorescence Microscope", Proceedings of the SPIE, in press.
    J.C. Meiners and S.R. Quake, "A Direct Measurement of the Hydrodynamic Interaction Between Two Particles", Phys Rev Lett 82: 2211-2214 (1999).
    J.P. Brody and S.R. Quake, "A Self-Assembled Microscopic  Rotational Probe", Appl Phys Lett. volume 74, no. 1, 4 January 1999.
     H.P. Chou, C. Spence, A. Scherer and S. Quake, "A Microfabricated Device for Sizing and Sorting DNA Molecules", Proc. Nat?l Acad. Sci. 96: 11-13 (1999).
    Pr RIGLER Rudolph
    Dept. of Medical Biophysics
    Karolinska Institute
    S-171 77 STOCKHOLM
    SWEDEN
    e-mail : rr@mango.mef.ki.se
    Pr  ROKHSAR Daniel
    Dept. of Physics
    University of California
    549 Birge Hall
    BERKELEY, CA 94720
    USA
    e-mail : rokhsar@marichal.berkeley.edu
    Folding pathway of a lattice model for proteins VIJAY S. PANDE AND DANIEL S. ROKHSAR PNAS . 96, pp. 1273--1278, (1999) 
    Is the molten globule a third phase of proteins? VIJAY S. PANDE AND DANIEL S. ROKHSAR  PNAS 95, pp. 1490--1494, (1998) 
    Pathways for protein folding: is a ``new view'' needed? 
    Vijay S Pande 1 , Alexander Yu Grosberg 2 , Toyoichi Tanaka 2 , and Daniel S Rokhsar 1;3 
    Folding pathway of a lattice model for protein folding 
    Vijay S. Pande 1 and Daniel S. Rokhsar 1;2 
    Pr  SCHENTER Greg
    Pacific Northwest National Laboratory
    MS K1-96, P.O.Box 999
    RICHLAND, WA 99352
    USA
    e-mail : Greg.Schenter@pnl.gov
    Pr  SCHMIDT Thomas
    Institute for Biophysics
    University of Linz
    Altenberg Strasse 69
    4040 LINZ
    AUSTRIA
    e-mail : Thomas.Schmidt@jk.uni-linz.ac.at
    Imaging of single molecule diffusion
    Th. Schmidt, G.J. Schütz, W. Baumgartner, H.J. Gruber and H. Schindler, PNAS, 93  p. 2926-2929; (1996).
    Pr  SELVIN Paul
    Dept. of Physics and Biophysics
    University of Illinois
    1110 W. Green St.
    URBANA, IL 61801
    USA
    e-mail : pselvin@physics.uiuc.edu
    Single Molecule Dynamics Studied by Polarization Modulation
    T. Ha, Th. Enderde, D.S. Chemla P.R. Selvin. and S. Wziss Phys. Rev. Lett. 77, 3979-3983; (1996)
    Pr SIEGELBAUM Steven A.
    Dept. of Pharmacology
    Columbia University
    NEW YORK,  NY 10032
    USA
    e-mail : sas8@columbia.edu
    Constraining Ligand-Binding Site Stoichiometry Suggests that a Cyclic Nucleotide-Gated Channel Is Composed of Two Functional Dimers
    D.T. Liu, G.R. Tibbs, P.Paoletti ans S. Siegelbaum Neuron 21, 1998 p 235-248
    G-Linker of Cyclic Nucleotide-gated Channels Controls Coupling of Ligand Binding to Channel Gating
    P. Paoletti, E. Young and S. A. Seigelbaum.  J.Gen.Physiol. 113 (1999) p17-33
    Pr SIGWORTH Fred
    Dept. of Cellular and Molecular Physiology
    Yale University
    NEW HAVEN, CT 06520
    USA
    e-mail : fred.sigworth@yale.edu
    Selectivity Changes during Activation of Mutant Shaker 
    Potassium Channels 
    Jie Zheng and Fred J. Sigworth;  J. Gen. Physiol 110 (1997), p 101--117 
    Pr  SPUDICH Jim A.
    Dept. of Biochemistry and Dev. Biology
    Beckman Center
    Stanford University
    STANFORD, CA 94305
    USA
    e-mail : jspudich@Cmgm.Stanford.Edu
    Mehta, A.D., Rief, M., Spudich, J.A., Smith, D.A., and Simmons, R.M. (1999) Single-molecule biomechanics with optical methods. Science283: 1689-1695. Mehta, A., Rief, M., and Spudich, J.A. (1999) Biomechanics, one molecule at a time. J. Biol. Chem. 274:xxx-xxx. Mehta, A.D., Rock, R.S., Rief, M., Spudich, J.A., Mooseker, M.S., and Cheney, R.E. (1999). Myosin V: A processive actin-based motor. Nature, submitted. 
    Pr VALE Ronald D.
    Cellular & Molecular Pharmacology
    University of California
    Box 0450, HSE 1020B 
    SAN FRANCISCO, CA 94143
    USA
    e-mail : vale@phy.ucsf.edu
    Imaging Individual GFP protein 
    D.W. Pierce, N. Hom-Booher and R.D. Vale, Nature 488 (1997), 338
    Direct Observation of Single Kinesin Molecules Moving along Microtubules
    R.D. Vale, T. Funatsu, D.W. Pierce, L. Romberg, Y. Harada and T.Yanagida Nature 380 (1996),451
    Pr  WEBB Watt W.
    Cornell University
    223 Clark Hall
    ITHACA, NY 14853
    USA
    e-mail : www2@cornell.edu
    Dynamics of fluorescence fluctuations in green fluorescent protein observed by 
    fluorescence correlation spectroscopy
    U. Haupts, S. Maiti, P. Schwille and W.W. Webb
    PNAS, 95, 13573-13578 (1998)

    Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules
    Richik N. Ghosh and W.W. Webb
    Biophys. J. 66, 1301-1318 (1994)

    Pr  WEISS Shimon
    Lawrence Berkeley National Laboratory
       Materials Sciences and Physical Biosciences 
     MS 2-300, 1 Cyclotron Rd.
    BERKELEY, CA 94720
    USA
      e-mail: sweiss@lbl.gov 
    Fluorescence Spectroscopy of Single Biomolecules
    S. Weiss, Science283 1676-1683, (1999)

    Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism
    T. Ha, A. Ting, J. Liang, W. B. Caldwell, A.A. Deniz, D.S. Chemla, P.G. Schultz and S. Weiss. PNAS, 96, p 893-898 (1999)

    Pr  XIE X. Sunney 
    Dept. of Chemistry and Chemical Biology
    Harvard University
    12, Oxford Street 
    CAMBRIDGE, MA 02138
    USA
    e-mail : xsxie@pnl.gov              xie@chemistry.harvard.edu
    Abstract : Single-Molecule enzymology
    Sunney X. Xie

    Single-Molecule enzymatic dynamics
    H. Peter Lu, Luying Xun, and X. Sunney Xie
    Science, 282, pp. 1877-1882 (1998)

     

    Pr  YANAGIDA Toshio
    Osaka University Medical School
    Dept. of Physiology I
    2-2 Yamadaoka
    OSAKA 565-0871
    JAPAN
    e-mail : yanagida@bpe.osaka-u.ac.jp
    Simultaneous Observation of Individual ATPase and Mechanical Events by a Single Myosin Molecule during Interaction with Actin
    A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka and T. Yanagida; Cell 92, (1998) p 161-171
    Pr  YEUNG Edward S.
    USDOE and Department of Chemistry
    Ames Laboratory
    Iowa State University
    AMES, IA 50011
    USA
    e-mail : yeung@ameslab.gov
    Direct Measurement of Single-Molecule Diffusion and Photodecomposition in Free Solution
    X. Xu and E.S. Yeung; Science, 275, p 1106-1109; (1997)
    Long-Range Electrostatic Trapping of Single-Protein Molecules at Liquid-Solid Interface
    X. Xu and E.S. Yeung; Science, 281, p. 1650-1653, (1998).

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    Contributed short communications

    If you want to print these short contributions as well as the posters, use this acrobat fileabtracts.pdf


    S. Allen, M. C. Davies, C. J. Roberts, Saul J.B. Tendler and P. M. Williams.
    Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences,
    The University of Nottingham, University Park, Nottingham, NG7 2RD.
    MEASUREMENTS OF BIOMOLECULAR INTERACTIONS IN PHARMACEUTICAL & IMMUNODIAGNOSTIC SYSTEMS
    Manfred Auer (1), Kurt A. Stoeckli (2), Karsten Gall (3) Werner Thumb (1), Carmen Barske (2), and Peet Kask (3) 
    (1) Novartis Forschungsinstitut, NFI, Vienna, Austria, (2) Novartis Pharma AG, Basel, Switzerland (3) EVOTEC Biosystems AG, Hamburg, Germany
    manfred.auer@pharma.novartis.com                                 www.at.novartis.com
    Interfacing Novel Detection Technologies in Single Molecule Spectroscopy with Drug Discovery.
    David M. Warshaw1, Eric Hayes1, Donald Gaffney1, Anne-Marie Lauzon1, Junru Wu2, Guy Kennedy3, Kathleen Trybus1, Susan Lowey1, and Christopher L. Berger1. Departments of 1Molecular Physiology and Biophysics, 2Physics, and the 3Instrumentation and Model Facility, University of Vermont, Burlington, VT 05405. Structural dynamics of myosin detected by single molecule fluorescence polarization.
    Eaton, William A.
    Laboratory of Chemical Physics, NIH, Bethesda, MD, USA
    eaton@helix.nih.gov 
    Kinetics and Mechanisms in Protein Folding
    Akihiko Ishijima
    NAGOYA University Chikusa-ku, Nagoya, 464-8603, JAPAN
    ishijima@nuap.nagoya-u.ac.jp
    Simultaneous Measurement of Individual ATPase and Mechanical Reactions by a Single Myosin  Molecule at Work
    T. Ha, H. Babcock, W. Cheng, T. Lohman and S. Chu 
    Stanford University and Washington University
    tjha@stanford.edu 
    Kinetics of Single DNA unwinding by Rep Helicase
    J. Hofkens, T. Gensch, F.C. De Schryver 
    Department of Chemistry, K.U.Leuven Celestijnenlaan 200 F Heverlee 3001-B Belgium Phone: +3216327414 , Fax: +3216327990 ohan.Hofkens@chem.kuleuven.ac.be
    SPECTROSCOPY AND MICROSCOPY OF A SINGLE MULTICHROMOPHORIC DENDRIMER MACROMOLECULE.
    Fritz Keilmann, 
    Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany
    keilmann@biochem.mpg.de
    VIBRATIONAL-INFRARED NEAR-FIELD MICROSCOPY
    David Keller*, Gijs Wuite ?, and Carlos Bustamante?
    *Dep. of Chemistry, University of New Mexico, Albuquerque, NM  87131
    ? University of California, Berkeley, CA 94720
    Mechanochemical Models for Molecular Motors
    Maier, Berenike
    Physikdepartment E22, TU-Muenchen, James-Franck-Strasse 1, D-85748 Garching LPS, ENS 24, rue Lhomond, 75005 Paris bmaier@physik.tu-muenchen.de
    Activity of DNA polymerase observed by elastic measurements on single DNA molecules
    Takayuki Nishizaka, K. Adachi, M. Yoshida and K. Kinosita Jr.
    taka@phys.keio.ac.jp
    Binding and hydrolysis of fluorescently labeled nucleotide by F1-ATPase.
    Owe Orwar, (Department of Chemistry, Goeteborg University Goeteborg, SE-41296, Sweden, ), D. T. Chiu, (Department of Chemistry, Harvard
    University,) & R. N. Zare (Department of Chemistry, Stanford University.)
    Chemical Transformations in Individual Ultrasmall
    Biomimetic Containers
    Pralle, Arnd
    Cell Biology and Biophysics, EMBL, Meyerhofstr. 1, 69117 Heidelberg 
    pralle@embl-heidelberg.de     www.embl-heidelberg.de/ExternalInfo/hoerber
    Local environment of single membrane proteins studied by 3D-thermal noise analysis
    M. Sauer
    Universität Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany
    sauer@sun0.urz.uni-heidelberg.de
    DETECTION, IDENTIFICATION AND HANDLING OF SINGLE BIOMOLECULES USING SEMICONDUCTOR LASERS AND MICROELECTROPHORESIS
    C. A. M. Seidel, C. Eggeling, S. Berger, E. Schweinberger, J. Schaffer,
    A. Volkmer, J. Fries, J. Widengren, G. Striker
    Multi-dimensional State-selective Fluorescence Spectroscopy 
    of Single DNA-Molecules in Solution
    Martin Hegner#, Steven B. Smith, and Carlos Bustamante
    Dept. of Molecular and Cell Biology, Univ. of California, Berkeley CA 94720, USA #new address: Dept. of Physics, Univ. of Basel, Switzerland
    Polymerization and Mechanical Properties of Single RecA-DNA Filaments
    T. Strick
    LPS-ENS 24 rue Lhomond 75231 Paris
    strick@clipper.ens.fr
    Single-molecule analysis of TopoisomeraseII/DNA interactions
    Uhl, Volker, Battulga Nasanshargal, Buerk Schaefer, Karl Otto Greulich 
    Institut fuer Molekulare Biotechnologie, Dept. Single Cell and Single Molecule Techniques, P.O.Box 100 813, D-07708 Jena, Germany 
    uhl@imb-jena.de                                                      www.imb-jena.de/greulich/
    From single molecule biophysics to single molecule biochemistry: Observation of reactions in the light microscope.
    Xiaowei Zhuang, T. Ha, H. Babcork, L. Bartley, R. Russell,
    S. Labeit, D. Herschlag, and S. Chu
    address  Department of Physics, Stanford University, Stanford, CA94305, USA
     zhuang@stanford.edu 
    Folding/unfolding of single protein and RNA molecules observed by fluorescence
    Giovanni Zocchi Plastic deformation of a protein 

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    Contributed poster communications

        The best poster will recieved a prize at the end of the workshop.
     
    GRÉGOIRE BONNET#, OLEG KRICHEVSKY#* AND ALBERT LIBCHABER#
    # Center for Studies in Physics and Biology, The Rockefeller University,
    1230 York Avenue, New York NY 10021, USA * LDFC, Institut de Physique, Université Louis Pasteur, 3 rue de l'Université, 67000 Strasbourg, France
    Kinetics of conformational fluctuations in DNA hairpin-loops
    Braslavsky Ido 
    Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel 
    febras@wicc.weizmann.ac.il        www.weizmann.ac.il/~febras
    Stretching and probing of single protein molecules: A study in structure-function  relationship 
    Zev Bryant1, Vijay Pande2, and Daniel Rokhsar2 
    Departments of 1Molecular and Cell Biology and 2Physics, 
    University of California at Berkeley, 
    Berkeley, CA 94720                    bryant@lsa.berkeley.edu 
    Unbending a hairpin:  molecular dynamics simulations of the mechanical unfolding of a model peptide.
    Henri Buc
    Unité de Physicochimie des Macromolécules Biologiques (URA1773 du CNRS)
    INSTITUT PASTEUR, 75724 PARIS CEDEX 15
    T : 01-4568 8504 - F : 01-4061 3060 - henribuc@pasteur.fr
    INITIATION OF TRANSCRIPTION. A COMPARISON BETWEEN TWO DNA-DEPENDENT RNA POLYMERASES.
    Chirico, Giuseppe, and Beretta, Sabrina,                                 Univ. Di Milano, Via Celoria 16, 20133, 
    Milano, (I)  giuseppe.chirico@mi.infm.it
    Study of the fluorescence fluctuation of highly dilute and semidilutesolutions by photon distribution method.
    COCCO Simona 
    Lab. de Physique ENS-Lyon, Lyon - France LPT-ENS, Paris - France
    scocco@physique.ens-lyon.fr
    A model for DNA base pairs opening 
    Y. Durand, A. Bloess, E.J.J. Groenen, J. Schmidt
    Leiden Univ. Huygens Lab.P.O. Box 9504, 2300 RA Leiden, The Netherlands
    Spectral diffusion of single hydrocarbons molecules in polycrystalline environment.
    Ezhkova Elena.Laboratory of  Biological Microchips, Engelhardt Institute of Molecular Biology
    Vavilov street 32 Moscow , 117984 RUSSIA
    Analysis of chromosome translocations, which  induce  chronic myeloid leukemia and their detection by means of hybridization on oligonucleotide microchips
    C. Faivre, M. Dogterom
    FOM Institute AMOLF Amsterdam, The Netherlands 
    About the role of microtubule assembly and force generation in the positioning of microtubule asters.
    Laura Finzi and Jeff Gelles, 
    Dip.di Biologia Universita' Statale di Milano Via Celoria 26 20133 Milano
    Laura.Finzi@unimi.it
    Lac repressor-mediated DNA loop formation and breakdown studied by single
    molecule microscopy
    J. Fritz*, M. K. Baller*, H.P. Lang*, M. Despont, U. Drechsler, H. Rothuizen, P. Vettiger, Ch. Gerber, J. K. Gimzewski, E. Meyer*, H.-J. Güntherodt*
    IBM Research Division, Zurich Research Laboratory, Säumerstrasse 4, CH-8803 Rüschlikon, Switzerland * Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
    Versatile Si Sensors for the Nano Scale
    Lori S. Goldner, Jeeseong Hwang, Kenneth Weston, Garnett Bryant
    National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA
    Phone: (301)975-3792  Fax: (301)840-8551 e-mail: lgoldner@nist.gov
    Organic Thin Films Studied by Near-Field Scanning Optical Microscopy 
    T. Ha, X. Zhuang, H. Kim , J. Orr, J. Williamson and S. Chu
    Stanford University and Scripps Institute
    tjha@stanford.edu 
    Ligand induced conformational changes of single RNA molecules
    Helen G. Hansma, Mario Viani, George T. Paloczi, Christine Chen, Lia I. Pietrasanta, and Paul K. Hansma
    Department of Physics, University of California, Santa Barbara, CA 93106
    Biomolecular processes and structures by AFM
    Frank Jülicher 
    Institut Curie Paris France
    jukicher@curie.fr
    Energy transduction and efficiency of molecular motors
    David Keller*, Gijs Wuite ?, and Carlos Bustamante?
    *Dep. of Chemistry, University of New Mexico, Albuquerque, NM  87131
    ? University of California, Berkeley, CA 94720
    Single-Molecule Mechanochemistry and Molecular Motors
    Jens-Christian Meiners and Stephen Quake 
    (Dept. of Applied Physics, California Institute of Technology, Pasadena, CA 91125.)
    Dynamics of Extended DNA Molecules
    Helim Aranda-Espinoza and Nily Dan (University of Delaware),
    Laurence Ramos (Université de Montpellier),
    Yi Chen, T. C. Lubensky, Philip Nelson(University of Pennsylvania)
    Positively Charged Vesicles Can Repel Negative Objects
    Takayuki Nishizaka, K. Adachi, M. Yoshida and K. Kinosita Jr.
    taka@phys.keio.ac.jp
    Binding and hydrolysis of fluorescently labeled nucleotide by F1-ATPase.
    Amit D. Mehta1, Matthias Rief1, Ronald S. Rock1, James A. Spudich1,
    Mark S. Mooseker2, Richard E. Cheney3
    1Department of Biochemistry Stanford University Medical Center
    2Department of Molecular, Cellular and Developmental Biology Yale University
    3University of North Carolina at Chapel Hill
    Myosin V is a processive actin based motor
    F. Daumas, C. Millot, L. Salomé et J.F. Tocanne
    IPBS-CNRS 118, route de Narbonne 31062 TOULOUSE cedex
    TRACKING OF A MEMBRANE RECEPTOR BY NANOVID MICROSCOPY
    K. S. Thorn, C. Hart and R. D. Vale
    Graduate Group in Biophysics and Howard Hughes Medical Institute,
    University of California San Francisco, San Francisco, CA 94122 USA
    Force production and processivity in mutant kinesins.
    C. Tietz1, F. Jelezko1, J. Schuster1, A. Schubert2, J. Wrachtrup1
    (1)Institute of Physics, University of Technology Chemnitz, 09107 Chemnitz
    (2)Institute of Biology, Humboldt University of Berlin, 12489 Berlin
    Investigation of energy transfer pathways in single plant antenna complexes
    G. J. L. Wuite1, R. J. Davenport2, R. Landick3, and C. J. Bustamante1,2
    1Physics  2 Molecular and Cell Biology, Univ.of California, Berkeley, CA 94720
    3Department of Bacteriology, University of Wisconsin, Madison, WI 53706
    Distinct Functional States of E.coli RNA Polymerase Affect its Pausing and 
    Elongation: A Single Molecules Study

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    Contact


    Braslavsky Ido 
    Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel 
    febras@wicc.weizmann.ac.il        www.weizmann.ac.il/~febras
    Measurments of single molecule enzymatic activity.
    I am looking for  a postdoctoral position
    Stretching and probing of single protein molecules: A study in structure-function  relationship 

    THEMES AND MAJOR OBJECTIVES OF THE WORKSHOP

    In the past 2-3 years a flurry of novel techniques and methods (optical and magnetic tweezers, two-photon excitation , fluorescence correlation spectroscopy, evanescent wave fluorescence microscopy,  near field scanning probe microscopy, etc.) have been applied to the manipulation, visualization and  study of single bio-molecules, thereby opening a new vista on the investigation of biological processes  at their most fundamental level. As a representative but non-exhaustive list, let us cite :

        1) The studies of Steve Chu's group on the relaxation dynamics of single DNA molecules (S.R.Quake.  et al., Nature 388, 151 (1997)).
        2) The observation of novel structures in overstitched DNA and their possible relevance to  DNA/protein interactions (in particular RecA) (P.Cluzel et al., Science 271, 792 (1996) and S.Smith  et al., Science 271, 795 (1996)).
        3) The characterization of single molecular motors, in particular myosin moving on actin or kinesin  (dynein) moving on microtubules (T.Funatsu et al., Nature 374, 555 (1995) and M.J.Schnitzer &  S.M.Block, Nature 388, 386 (1997)).
        4) The investigation by the groups of S.Block and J.Gelles of yet an other fundamental motor : a single  RNA-polymerase moving along a single DNA molecule (H.Yin et al., Science 270, 1653 (1995)).
        5) The observation and study by the group of Kinosita of the rotation of the F1-ATPase rotor (H.Noji  et al., Nature 386, 295 (1997)).
        6) The measurement of the interaction force between a single molecule and its ligand (E.-L. Florin et  al., Science 264, 415 (1994)).
        7) The observation of conformational transitions on single molecules in solution (L.Edman et al.,  PNAS 93, 6710 (1996)).
        8) The study of the fluorescence emission by single molecules, in particular the biologically relevant  GFP (D.A. Vanden Bout et al., Science 277, 1074 (1997) & R.M. Dickson et al. , Nature 388, 355  (1997)).
        9) The observation of the quantal release of neuro transmitters by a single synaptic vesicle (J.A.Steyer  et al., Nature 388, 474 (1997) & T.A.Ryan et al., Nature 388, 478 (1997)).

    As evidenced by this non-exhaustive list, Single Molecule Biophysics is a very active and exciting field. It requires an interdisciplinary mastery of a number of state of the art techniques and  methods. From the physics side one needs to perform micro-manipulation on single molecules with  optical or magnetic tweezers or some adapted near field probes. One also needs to visualize single  molecules and study their fluorescence spectra. Hence the need to master new techniques such as two  photon fluorescence excitation, evanescent wave illumination, fluorescence correlation spectroscopy and low noise/low background photon detection. From the biological side one needs to have full control and characterization of the bio-molecules under study (protein purification and characterization (enzymatic activity, crystallography, NMR, etc.), site directed mutagenesis, protein fusions, etc.) . Finally from a chemical point of view there is a strong incentive to develop new reactive groups to label bio-molecules of interest at specific places.

    This large array of techniques is used to address a number of fundamental problems.

        1) To study the function of bio-molecules by manipulating them individually, for example measuring  the force required to stall a single RNA-polymerase on its DNA tracks (see example 4 in the preceding list).
        2) To study and induce structural transitions in bio-molecules as exemplified in the DNA studies mentioned previously (example 2 above).
        3) To increase the spatial and temporal resolution in the study of biological processes. In that respect  the measurement of the step size of myosin and of F1-ATPase and of the number of hydrolyzed ATP per step are exemplary (examples 3 and 5 above).
        4) To see how the thermodynamic properties commonly measured on a large molecular population  reflect the observed behavior of a single molecule. Here the work of Gaub relating the binding force of a single biotin/streptavidin link to the thermodynamic enthalpy raises fascinating questions.
        5) The theoretical description of these molecular « engines » requires new concepts (e.g. thermal ratchets) which differ radically from the ones used to study macroscopic engines (e.g. Carnot cycle). The work of J.Prost and his co-workers is a nice example of this new approach.

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    Evaluation procedure used for candidate registration

        To evaluate the 125 candidates, we have separate them in two categories : "new in the field" and "experts" (you are an "expert" if you have already published a paper in the field). For each candidate, we have then elected two speakers acting as referee of their preregistration form. Each speaker has reviewed typically eight persons. While the organizers have reviewed all candidates to replace the unreachable speakers and to compensate for the difference between speakers. We wish to thank the speakers for their quick answer, to a few exceptions they have been able to perform this review. From these evaluations we have selected the accepted candidates, we have also taken in account the number of participant coming from the same university, a balance between the nationality (as required by EMBO), and the fact that the candidate was a student or postdoc). Thus 15 candidates will participate as new in the field and 45 as experts. We have asked R. Lavery and J. Prost to review our evaluation procedure. This process took two weeks and some intense work of all the speakers and organizers.
     

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    You are depressed because you have not been selected

    Having read all the preregistration forms we know that we have not accepted very motivated people and we truly apologize for this inconvenience. We have received very well argumented messages to change our choice and we understand that for all of you this conference represents a real opportunity.

    To justify our choice let us explain the reasons : when we started organising this workshop -which is the first real one concerning Single Molecule Biophysics- we thought that this was an emerging field and that a one hundred people workshop was the right scale (it is also the EMBO scale). As soon as we started receiving the preregistration forms it appeared that the community was larger than expected. We tried  to see if it was possible to increase the number of participants. The main limitation comes from the lecture room. Other lecture rooms were proposed in Tours. Unfortunately, when we visited them they appeared to be either not large enough or too far from "La Croix Montoire" where we had already reservations.

    We were thus left with the difficult choice of selecting one participant for every two applicants. As explained above, this evaluation was made mainly by the speakers. It is clear that the preregistration form has played a major role in this process. The overall level of the applicants was very high, and the fact that you are not selected does not mean anything regarding your work. It is clear that many applicants starting their activity in the field of single molecule biophysics have not been selected only because we have underestimated the size of the community. If there is another SMB conference later, it should be scale properly so that basically all applicants could participate.

        This also means that if you have been selected as participant and cannot come to a part or the entire workshop,
       please consider giving your position to someone on the waiting list.

        Meanwhile we propose to open the conference for exchange between the people in the community : we shall post at the conference inquiries of applicants looking for postdoc, collaborations infos etc. In this idea, you can send us a contact page that we shall link to this page.

        Depending on Tours facilities, we are thinking of placing transparencies on the web so that you may acces them.

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