You can also become a sponsor of this workshop by contacting us.
This EMBO workshop will be held in Tours
(Loire valley, France )
between the 8th and 15th of July 1999. It will gather about
one hundred physicists, chemists and biologists studying single molecule
biophysics. The purpose of this workshop is to gather the specialists in
this field together with interested students. Lectures will first describe
the different techniques involved in single molecule experiments (optical
and magnetic tweezers, two-photon excitation, fluorescence correlation
spectroscopy, evanescent wave fluorescence microscopy, near field
scanning probe microscopy, etc..) and then will focus on more specialized
topics (DNA, molecular motors, single enzymatic reactions etc...).
A.Adams, P. Barbara, H. Berg,
D. Branton, C. Bustamante, H.Gaub, J. Gelles, Y.Goldman,
F. Heslot, E. Isacoff, P. Hansma,
R. Hochstrasser, K. Kinosita,
S. Lindsay, J. Marko, M.Orrit, O.Orwar, J. Prost, S. Quake, R. Rigler,
D. Rokhsar, G. Schenter,
T. Schmidt, P. Selvin, S.
Siegelbaum, F. Sigworth, J. Spudich, R. Vale, W.W. Webb, X.S.
Xie, T. Yanagida, E.S. Young.
The workshop will be held from July 8 in the morning to July 15 in the afternoon in a XIX century Manor at :
Domaine de "la Croix Montoire"
8 bis place Choiseul
37 100 TOURS
(FRANCE)
Tel. : 33 -2 47 42 57 68
Fax : 33- 2 47 49 18 32
Participants are expected to arrive on July 7 and register at the SMB office in the Manor, starting 2 PM.
Both young researchers (student and postdocs) and specialists applied to this workshop. The deadline for the registration was April 25th and we were more than twice oversubscribed. The selection of the candidates relied upon the speakers acting as referees of the candidates in their field and evaluating their participation on the basis of their work and letter of intent .
Participants will be housed in
student accommodation located in the manor i.e. in individual rooms
with collective facilities. Breakfast and lunch will be served
in the manor. Participants will be free to have dinner either
in the center of TOURS (five minute walk) or use the common kitchen.
A4 or Letter size poster in Acrobat file format (.pdf)
Arriving by car, Tours is connected from Paris by the A10 high way until you see the sign Tours center, you will find here a general map for Tours area, a general road map, a city center road map
A detailed Tours web site with various maps (in french)
Travel arrangements should be made by the participants who are expected to arrive on July 7 and leave on July 15 in the afternoon. Please notice that Tours is located south from Paris. It is within one hour from Paris by fast train (TGV). The name of the TGV train station near Tours is Saint Pierre des Corps. Driving from Paris to Tours is also possible but won't be so fast (3 hours). We recommend that as soon as you receive our agreement for your participation to the conference, you purchase a plane and train ticket for Tours.
You can find a schematic map of Paris area explaining the access between airports and train station
To buy a RER ticket at the teller you can use one of these french forms
Paris has two airports : Charles de Gaulle (Roissy) in the north and Orly in the south. Most international flights arrive in Charles de Gaulle (CDG). TGV Trains for Tours depart either from Massy-TGV station (near Orly), CDG-TGV station (inside CDG airport) or from Montparnasse Station (downtown Paris). On July the 7th, there are five trains starting from CDG-TGV, whereas there are nine starting Massy-TGV station and fifteen departing from Montparnasse station. We list below the timetable for the three stations. Depending on your schedule you should check the optimal solution taking into account that connection between CDG and Massy takes 1h00 but is direct by RER-B, CDG to Montparnasse station takes 1h15 but requires taking RER-B and the subway, Orly to Massy-TGV takes 0h20 and is very convenient. Your choice will probably be dictated by your flight's arrival time. If you can choose the airport when purchasing your flight tickets, choose Orly.
Trains departing from Paris and arriving in Tours
Be aware that the TGV station of Tours is located at a few kilometers from downtown Tours. The name of this TGV station is Saint Pierre des Corps. At this station, you have to leave the TGV and take a normal train which will take you to Tours downtown station. The connection is done for all TGVs and is very quick (5 or 10 minutes). This correspondance is announced (in french) when the TGV arrives, it is also indicated on panels and is quite easy. However if you have to purchase your TGV ticket you might have to indicate that your destination is Saint Pierre des Corps.
When you take the TGV, you must have your ticket punched by a red machine BEFORE you get on the train. Don't forget to do it otherwhise, you will have to pay an extra fee on the train!
To buy a TGV ticket at the teller you can use one of these french forms
From Roissy (95) to Tours on July 7th
Charles de Gaulle TGV station | Saint Pierre des Corps (connection to Tours) |
7.45 am | 9.20 am |
8.55 am | 10.35 am |
1.45 pm | 3.20 pm |
6.54 pm | 8.43 pm |
9.29 pm | 11.06pm |
From Massy TGV (91) to Tours on July 7th
Massy TGV station | Saint Pierre des Corps (connection to Tours) |
7.02 am | 7.54 am |
8.31 am | 9.20 am |
8.56 am | 9.47 am |
9.46 am | 19.35 am |
2.31 pm | 3.20 pm |
3.49 pm | 4.39 pm |
7.48 pm | 8.43 pm |
8.47 pm | 9.37 pm |
1O.14 pm | 11.06 pm |
From Paris downtown to Tours on July 7th
Montparnasse TGV station | Saint Pierre des Corps (connection to Tours) |
6.50 am | 7.54 am |
7.40 am | 8.43 am |
9.05 am | 10.01 am |
10.45 am | 11.41 am |
12.35 am | 13.27 am |
2.05 pm | 3.01 pm |
3.25 pm | 4.23 pm |
4.35 pm | 5.31 pm |
5.15 pm | 6.11 pm |
5.40 pm | 6.36 pm |
6.05 pm | 7.09 pm |
6.40 pm | 7.42 pm |
6.50 pm | 7.46 pm |
7.35 pm | 8.38 pm |
10.15 pm | 11.12 pm |
To buy a RER ticket at the teller you can use one of these french forms
From Orly to Massy-TGV station, the fastest and easiest connection is to take the ORLYVAL to Antony (RER-B station and terminus of OrlyVal) and then take the RER-B southwards (opposite to Paris direction) heading to St Rémy-les-Chevreuses or Orsay or Massy-Palaiseau. Buy your ticket in Orly directly to Massy-Palaiseau (approx 50FF or 8$). All RER-B trains stop in Antony and in Massy-Palaiseau where you should leave the RER-B train. The Massy-TGV station and the Massy-Palaiseau RER-B station are very close (200 meters). Look for the sign MASSY-TGV.
From Charles de Gaulle (Roissy) to Massy-TGV station, After purchasing a ticket to Massy-Palaiseau (approx 50FF), take the RER-B train directly inside Roissy airport, check that the train actually stops in Massy-Palaiseau (the panel on the platform must mention Massy-Palaiseau). Get out of the RER-B train in Massy-Palaiseau. The Massy-TGV station and the Massy-Palaiseau RER-B station are very close (200 meters). Look for the sign MASSY-TGV.
From Charles de Gaulle (Roissy)
to Montparnasse TGV station : buy a RER ticket for Paris downtown, it will
cover both RER and subway trip. Take the RER-B and stop at the station
named Denfert-Rochereau. Follow the orange sign indicating "correspondance"
to acces directly to the subway. You should look for the "Nation/Etoile"
line 6, take the subway in the direction of Etoile. After a few stops,
you should reach "Montparnasse Bienvenue" subway station where you leave
the subway. Look then for the sign indicating Montparnasse SNCF train station.
After some corridors you should arrive to the TGV station.
Paris subway map Paris subway and RER map Paris RER map Paris ground transportation agency (RATP)
You should make your TGV reservation in advance (You can purchase your ticket with your credit card by calling 33 -836 35 35 39, an english speaking operator should answer you. You should then be able to withdraw your ticket in the train station on teller just by giving a code number provided by the operator). Your final destination is "Tours centre ville" (Tours center). You will be able to get the corresponding ticket in any Railway station (SNCF) in particular in Massy-TGV.
Arriving in Tours , the workshop location
(Domaine de la Croix-Montoire) can be joined by bus from both Tours railway
stations:
- Saint-Pierre des Corps: the TGV railway station, (nearly
all TGV have a corresponding train connecting them to Tours central Station)
- Tours centre. (central rail station)
From Saint-Pierre des Corps, take
bus line 3 and change at Place Jean Jaurès. Then take bus
lines 1, 2, 8, 9 or 13 northwards to the avenue de la Tranchée.
Once the Loire river is crossed, get out. The gate to the Domaine de la
Croix-Montoire is nearby. (Tours city map, downtown
map) To bget a french taxi you can use this french
forms
From Tours centre, take
bus lines 1, 2, 8, 9 or 13 northwards to the avenue de la Tranchée.
Once the Loire river is crossed, get out. The gate to the Domaine de la
Croix-Montoire is nearby.
The registration fees is 2000
FF for french participants and $400 for others; grants are available.
If you have received a message explaining
that we accept your participation to the workshop, you should pay your
registration by sending us a Fax with your name, the type (VISA, MC) and
number of your credit card, the expiration date and a sentence saying that
you accept that we debit your account of the corresponding amount.
ed to
Vincent.Croquette@physique.ens.fr.
The Deadline for accepted participants
to confirm their participation by paying their registration is May 30th,
1999.
After this date,
we shall propose unconfirmed position to those on the waiting list.
NAME AND ADDRESS OF ORGANIZERS
David Bensimon 1, Vincent Croquette,1 Ludovic Jullien 2and Shimon Weiss,3
If you want to print the programme, use this acrobat
file :smallprog.pdf
Thursday 8th | ||
8.30 am to 1 pm | Micromanipulation techniques: Optical /Magnetic tweezers, AFM | S. Quake, V. Croquette, P. Hansma, S. Lindsay |
2.30 pm to 5.30 pm | Guided visit of old Tours | |
6 pm to 7 pm | Single molecule fluorescence | F. Keilman, C.A.M. Seidel |
7.30 pm to 9 pm | Cocktail at the city Hall of Tours | |
Friday 9th | ||
8.30 am to 1 pm | Single molecule fluorescence/ ionic channels | S. Weiss, M. Orrit, E.Isacoff, F.Sigworth |
2pm to 5 pm | Practical workshop on micromanipulation | Posters , demos and discussions |
5 pm to 7.30 pm | ionic channels | P.Selvin, S.Sieglelbaum,A.Pralle |
Saturday 10th | ||
8.30 am to 1 pm | Single molecule at work Ligand / receptor interaction enzymatic activity | H.Gaub, S.Xie, E.Yeung, Wennalm S., G. Bonnet |
2 pm to 5 pm | Posters, demos and discussions | |
5 pm to 7.30 pm | Protein folding and structure | R.Hochstrasser, D.Rokhsar,W.A. Eaton |
Sunday 11th | ||
8.30 to 1pm | Protein folding and structure/ Photo-physics on single molecules | T.Schmidt, P.Barbara, S.Adams, G.Schenter |
2 pm to 5 pm | Posters, demos and discussions | |
5 pm to 7.30 pm | Protein folding and structure/ Photo-physics on single molecules | Y.Goldman,J.Hofkens, X.Zhuang, G.Zocchi |
Monday 12th | ||
8.30 am to 1 pm | Molecular motors | H.Berg, K.Kinoshita, J.Spudich,L.Berger, A. Ishijima |
2 pm to 4.pm | Posters, demos and discussions | |
4 pm to 6 pm | Molecular motors | T.Yanagida, R.Vale |
6.30 pm to 11 pm | Visit of Montlouis cellar and dinner | |
Tuesday 13th | ||
8.30 am to 1 pm | Single molecule technologies | O.Orwar, S.Allen, M.Auer, M.Sauer, V. Uhl., D.Branton |
2 pm to 10 pm | Social event: visit of Chenonceaux and Amboise | |
10 pm | Tours by night | |
Wednesday 14th | ||
8.30 am to 1 pm | Theory of molecular motors DNA structures | J.Prost, J. Marko, F.Heslot, R.Lavery, |
2 pm to 5 pm | Posters, demos and discussions | |
5 pm to 7.30 pm | DNA /Prospectives | S.B.Smith, round table |
8pm to next morning | Celebration of Bastille day | Firework and dancing |
Thursday 15th | ||
8.30 am to 1. pm | DNA/protein interactions | C.Bustamante, J.Gelles, T.Ha, B.Maier, T.Strick,D.Keller |
12.30 pm to 2 pm | Lunch | |
afternoon | departure |
Detailed Programme
If you want to print the programme, use this acrobat file :programme.pdf
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ADAMS Stephen
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« Targeting
of fluorescent indicators in living cells »
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BARBARA Paul
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« Energy funnels, folded conformations
and energy transfer domains in Single onjugated polymer molecules ».
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BERG Howard
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« Physiology
of the flagellar rotary motor »
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BRANTON Daniel
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« Prospects
for nanopore sequencing »
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BUSTAMANTE Carlos
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« Following
transcription one molecule at a time »
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CROQUETTE Vincent
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« Magnetic
tweezers and DNA supercoiling »
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GAUB Herman
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« Probing
chemical bonds by Single-Molecule force spectroscopy »
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GELLES Jeff
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« Studying
motor enzymes one molecule at a time »
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GOLDMAN Yale
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« Single-Molecule
structural dynamics by fluorescence polarization microscopy »
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HANSMA Paul
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« Single-Molecule
science with scanning probes »
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HESLOT François
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« Time resolved
force measurement on the opening and closing of DNA »
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HOCHSTRASSER Robin
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« Folding
of GCN-4-fs on silica and structures of single LH2 complexes »
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ISACOFF Ehud
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« Optical
measurement of ion channel gating rearrangements »
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KINOSITA Kazuhiko
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« Huge and
small tags for Single-Molecule physiology »
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LAVERY Richard
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« Modeling
studies linking DNA mechanics, dynamics and recognition »
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LINDSAY Stuart
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« Dynamic
force microscope for imaging and manipulating single molecules at the liquid-solid
interface »
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MARKO John
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« Structure,
Viscoelasticity and Plasticity of Mitotic Chromosomes »
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ORRIT Michel
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« Optical
detection and study of Single-Molecules »
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ORWAR Owe
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« Biochemical
reaction dynamics in ultra small biomimetic containers »
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PROST Jacques
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« From single
to many molecular motors »
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QUAKE Steven
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« Imaging
information in DNA- from base pairs to genomic dynamics »
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RIGLER Rudolph
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« Enzyme
catalysis at the Single Molecule level »
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ROKHSAR Daniel
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« Transition
states and intermediates in Single-Molecule models for protein folding
»
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SCHENTER Greg
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« Statistical
analysis of Single-Molecule enzymatic dynamics »
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SCHMIDT Thomas
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« Dynamical
behaviour of membrane components »
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SELVIN Paul
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« Voltage-induced movement in
a potassium ion channel: detection by ensemble energy transfer and the
potential of single-molecule »
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SIEGELBAUM Steven
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« Contribution
of individual ligand binding events to the activation of cyclic nucleotid-gated
channels » (leaves 13-14)
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SIGWORTH Fred
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« Intermediate
steps in the activation of voltage gated potassium channels » (leaves
14)
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SPUDICH Jim
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« Single-Molecule
biomechanics : the myosin family of molecular motors »
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VALE Ronald
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« Tracking
single molecule kinesin motility by fluorescence microscopy »
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WEBB Watt
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« Fast broad
band dynamics for Single-Molecules »
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WEISS Shimon
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« Observing
Single Molecule reactions »
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XIE X. Sunney
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« Single-Molecule
enzymology »
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YANAGIDA Toshio
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« Single
-Molecule imaging and nanomanipulation of molecular motors at work »
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YEUNG Edward
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« Characterization
of Single-Molecules in microenvironments »
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To prepare this workshop, you can already
improve your knowledge by reading some (or all) the articles listed below.
These articles have been given by the speakers in order to support their
talk. They are available in Acrobat format (*.pdf) by clicking on their
title.(This part is not yet finished !)
We stongly encourage you to prepare the oral presentation by writting your transparencies using a WEB composer (such as Netscape or Explorer which are both free). To use the composer mode, select this mode as you open a blank page in your web navigator. You can easily write a transparency with big titles, colors etc using standart word processor facilities. You can include graphics and drawing provide you have save them in GIF format. You can also include images and pictures using the JPEG format. Please do not include exterior link, we cannot guarantee the web connection. By preparing your presentation in this HTML format, you will allow us to put your seminar on the web for those that have not had the chance to participate. You can even help us more by preparing an archive of your seminar (containing the HTML files and all the linked material) in either ZIP or gzip format and by mailing it to Vincent.Croquette@physique.ens.fr in advance. Otherwise bring it on a zip disquette (PC format). We shall have a slide projector, a transparency overhead projector, a LCD video and computer projector accepting 640x480, 800x600 and 1024x768 modes. Our VCR support NTSC (american standart) and CCIR (european standart) VHS tape. Note however, we cannot guarantee that your tape or your computer will be fully compatible with our material, it is thus a good practice to make a few transparencies of your video or your computer presentation in case of trouble.
If your presentation rely upon SLIDES, beaware that we shall not have a way to digitize them in Tours ! (you will be able to project them but we shall copy them for the web page). Please have your slides digitized on PC zip disquettes.
If you do not want to have your talk on the web, just
inform us.
S. Allen, M. C. Davies, C. J. Roberts,
Saul J.B. Tendler and P. M. Williams.
Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD. |
MEASUREMENTS OF BIOMOLECULAR INTERACTIONS IN PHARMACEUTICAL & IMMUNODIAGNOSTIC SYSTEMS |
Manfred Auer (1), Kurt A. Stoeckli (2),
Karsten Gall (3) Werner Thumb (1), Carmen Barske (2), and Peet Kask (3)
(1) Novartis Forschungsinstitut, NFI, Vienna, Austria, (2) Novartis Pharma AG, Basel, Switzerland (3) EVOTEC Biosystems AG, Hamburg, Germany manfred.auer@pharma.novartis.com www.at.novartis.com |
Interfacing Novel Detection Technologies in Single Molecule Spectroscopy with Drug Discovery. |
David M. Warshaw1, Eric Hayes1, Donald Gaffney1, Anne-Marie Lauzon1, Junru Wu2, Guy Kennedy3, Kathleen Trybus1, Susan Lowey1, and Christopher L. Berger1. Departments of 1Molecular Physiology and Biophysics, 2Physics, and the 3Instrumentation and Model Facility, University of Vermont, Burlington, VT 05405. | Structural dynamics of myosin detected by single molecule fluorescence polarization. |
Eaton, William A.
Laboratory of Chemical Physics, NIH, Bethesda, MD, USA eaton@helix.nih.gov |
Kinetics and Mechanisms in Protein Folding |
Akihiko Ishijima
NAGOYA University Chikusa-ku, Nagoya, 464-8603, JAPAN ishijima@nuap.nagoya-u.ac.jp |
Simultaneous Measurement of Individual ATPase and Mechanical Reactions by a Single Myosin Molecule at Work |
T. Ha, H. Babcock, W. Cheng, T. Lohman and S.
Chu
Stanford University and Washington University tjha@stanford.edu |
Kinetics of Single DNA unwinding by Rep Helicase |
J. Hofkens, T. Gensch, F.C. De Schryver
Department of Chemistry, K.U.Leuven Celestijnenlaan 200 F Heverlee 3001-B Belgium Phone: +3216327414 , Fax: +3216327990 ohan.Hofkens@chem.kuleuven.ac.be |
SPECTROSCOPY AND MICROSCOPY OF A SINGLE MULTICHROMOPHORIC DENDRIMER MACROMOLECULE. |
Fritz Keilmann,
Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany keilmann@biochem.mpg.de |
VIBRATIONAL-INFRARED NEAR-FIELD MICROSCOPY |
David Keller*, Gijs Wuite ?, and Carlos
Bustamante?
*Dep. of Chemistry, University of New Mexico, Albuquerque, NM 87131 ? University of California, Berkeley, CA 94720 |
Mechanochemical Models for Molecular Motors |
Maier, Berenike
Physikdepartment E22, TU-Muenchen, James-Franck-Strasse 1, D-85748 Garching LPS, ENS 24, rue Lhomond, 75005 Paris bmaier@physik.tu-muenchen.de |
Activity of DNA polymerase observed by elastic measurements on single DNA molecules |
Takayuki Nishizaka, K. Adachi, M. Yoshida
and K. Kinosita Jr.
taka@phys.keio.ac.jp |
Binding and hydrolysis of fluorescently labeled nucleotide by F1-ATPase. |
Owe Orwar, (Department of Chemistry,
Goeteborg University Goeteborg, SE-41296, Sweden, ), D.
T. Chiu, (Department of Chemistry, Harvard
University,) & R. N. Zare (Department of Chemistry, Stanford University.) |
Chemical Transformations in Individual
Ultrasmall
Biomimetic Containers |
Pralle, Arnd
Cell Biology and Biophysics, EMBL, Meyerhofstr. 1, 69117 Heidelberg pralle@embl-heidelberg.de www.embl-heidelberg.de/ExternalInfo/hoerber |
Local environment of single membrane proteins studied by 3D-thermal noise analysis |
M. Sauer
Universität Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany sauer@sun0.urz.uni-heidelberg.de |
DETECTION, IDENTIFICATION AND HANDLING OF SINGLE BIOMOLECULES USING SEMICONDUCTOR LASERS AND MICROELECTROPHORESIS |
C. A. M. Seidel, C. Eggeling, S. Berger,
E. Schweinberger, J. Schaffer,
A. Volkmer, J. Fries, J. Widengren, G. Striker |
Multi-dimensional State-selective Fluorescence
Spectroscopy
of Single DNA-Molecules in Solution |
Martin Hegner#,
Steven B. Smith, and Carlos
Bustamante
Dept. of Molecular and Cell Biology, Univ. of California, Berkeley CA 94720, USA #new address: Dept. of Physics, Univ. of Basel, Switzerland |
Polymerization and Mechanical Properties of Single RecA-DNA Filaments |
T. Strick
LPS-ENS 24 rue Lhomond 75231 Paris strick@clipper.ens.fr |
Single-molecule analysis of TopoisomeraseII/DNA interactions |
Uhl, Volker, Battulga Nasanshargal, Buerk
Schaefer, Karl Otto Greulich
Institut fuer Molekulare Biotechnologie, Dept. Single Cell and Single Molecule Techniques, P.O.Box 100 813, D-07708 Jena, Germany uhl@imb-jena.de www.imb-jena.de/greulich/ |
From single molecule biophysics to single molecule biochemistry: Observation of reactions in the light microscope. |
Xiaowei Zhuang, T. Ha, H. Babcork, L.
Bartley, R. Russell,
S. Labeit, D. Herschlag, and S. Chu address Department of Physics, Stanford University, Stanford, CA94305, USA zhuang@stanford.edu |
Folding/unfolding of single protein and RNA molecules observed by fluorescence |
Giovanni Zocchi | Plastic deformation of a protein |
The best poster will recieved a prize
at the end of the workshop.
GRÉGOIRE BONNET#, OLEG KRICHEVSKY#*
AND ALBERT LIBCHABER#
# Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York NY 10021, USA * LDFC, Institut de Physique, Université Louis Pasteur, 3 rue de l'Université, 67000 Strasbourg, France |
Kinetics of conformational fluctuations in DNA hairpin-loops |
Braslavsky Ido
Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel febras@wicc.weizmann.ac.il www.weizmann.ac.il/~febras |
Stretching and probing of single protein molecules: A study in structure-function relationship |
Zev Bryant1, Vijay Pande2, and Daniel Rokhsar2
Departments of 1Molecular and Cell Biology and 2Physics, University of California at Berkeley, Berkeley, CA 94720 bryant@lsa.berkeley.edu |
Unbending a hairpin: molecular dynamics simulations of the mechanical unfolding of a model peptide. |
Henri Buc
Unité de Physicochimie des Macromolécules Biologiques (URA1773 du CNRS) INSTITUT PASTEUR, 75724 PARIS CEDEX 15 T : 01-4568 8504 - F : 01-4061 3060 - henribuc@pasteur.fr |
INITIATION OF TRANSCRIPTION. A COMPARISON BETWEEN TWO DNA-DEPENDENT RNA POLYMERASES. |
Chirico, Giuseppe, and Beretta, Sabrina,
Univ. Di Milano, Via Celoria 16, 20133,
Milano, (I) giuseppe.chirico@mi.infm.it |
Study of the fluorescence fluctuation of highly dilute and semidilutesolutions by photon distribution method. |
COCCO Simona
Lab. de Physique ENS-Lyon, Lyon - France LPT-ENS, Paris - France scocco@physique.ens-lyon.fr |
A model for DNA base pairs opening |
Y. Durand, A. Bloess,
E.J.J. Groenen, J. Schmidt
Leiden Univ. Huygens Lab.P.O. Box 9504, 2300 RA Leiden, The Netherlands |
Spectral diffusion of single hydrocarbons molecules in polycrystalline environment. |
Ezhkova Elena.Laboratory of Biological
Microchips, Engelhardt Institute of Molecular Biology
Vavilov street 32 Moscow , 117984 RUSSIA |
Analysis of chromosome translocations, which induce chronic myeloid leukemia and their detection by means of hybridization on oligonucleotide microchips |
C. Faivre, M. Dogterom
FOM Institute AMOLF Amsterdam, The Netherlands |
About the role of microtubule assembly and force generation in the positioning of microtubule asters. |
Laura Finzi and Jeff Gelles,
Dip.di Biologia Universita' Statale di Milano Via Celoria 26 20133 Milano Laura.Finzi@unimi.it |
Lac repressor-mediated DNA loop formation
and breakdown studied by single
molecule microscopy |
J. Fritz*, M. K. Baller*,
H.P. Lang*, M. Despont, U. Drechsler, H. Rothuizen, P. Vettiger,
Ch. Gerber, J. K. Gimzewski, E. Meyer*, H.-J. Güntherodt*
IBM Research Division, Zurich Research Laboratory, Säumerstrasse 4, CH-8803 Rüschlikon, Switzerland * Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland |
Versatile Si Sensors for the Nano Scale |
Lori S. Goldner, Jeeseong Hwang, Kenneth
Weston, Garnett Bryant
National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA Phone: (301)975-3792 Fax: (301)840-8551 e-mail: lgoldner@nist.gov |
Organic Thin Films Studied by Near-Field Scanning Optical Microscopy |
T. Ha, X. Zhuang, H. Kim , J. Orr, J.
Williamson and S. Chu
Stanford University and Scripps Institute tjha@stanford.edu |
Ligand induced conformational changes of single RNA molecules |
Helen G. Hansma, Mario Viani, George T.
Paloczi, Christine Chen, Lia I. Pietrasanta, and Paul K. Hansma
Department of Physics, University of California, Santa Barbara, CA 93106 |
Biomolecular processes and structures by AFM |
Frank Jülicher
Institut Curie Paris France jukicher@curie.fr |
Energy transduction and efficiency of molecular motors |
David Keller*, Gijs Wuite ?, and Carlos
Bustamante?
*Dep. of Chemistry, University of New Mexico, Albuquerque, NM 87131 ? University of California, Berkeley, CA 94720 |
Single-Molecule Mechanochemistry and Molecular Motors |
Jens-Christian Meiners and Stephen Quake
(Dept. of Applied Physics, California Institute of Technology, Pasadena, CA 91125.) |
Dynamics of Extended DNA Molecules |
Helim Aranda-Espinoza and Nily Dan (University
of Delaware),
Laurence Ramos (Université de Montpellier), Yi Chen, T. C. Lubensky, Philip Nelson(University of Pennsylvania) |
Positively Charged Vesicles Can Repel Negative Objects |
Takayuki Nishizaka, K. Adachi, M. Yoshida
and K. Kinosita Jr.
taka@phys.keio.ac.jp |
Binding and hydrolysis of fluorescently labeled nucleotide by F1-ATPase. |
Amit D. Mehta1, Matthias
Rief1, Ronald S. Rock1, James A. Spudich1,
Mark S. Mooseker2, Richard E. Cheney3 1Department of Biochemistry Stanford University Medical Center 2Department of Molecular, Cellular and Developmental Biology Yale University 3University of North Carolina at Chapel Hill |
Myosin V is a processive actin based motor |
F. Daumas, C. Millot, L. Salomé et
J.F. Tocanne
IPBS-CNRS 118, route de Narbonne 31062 TOULOUSE cedex |
TRACKING OF A MEMBRANE RECEPTOR BY NANOVID MICROSCOPY |
K. S. Thorn, C. Hart and R. D. Vale
Graduate Group in Biophysics and Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94122 USA |
Force production and processivity in mutant kinesins. |
C. Tietz1, F. Jelezko1,
J. Schuster1, A. Schubert2, J. Wrachtrup1
(1)Institute of Physics, University of Technology Chemnitz, 09107 Chemnitz (2)Institute of Biology, Humboldt University of Berlin, 12489 Berlin |
Investigation of energy transfer pathways in single plant antenna complexes |
G. J. L. Wuite1, R. J. Davenport2,
R. Landick3, and C. J. Bustamante1,2
1Physics 2 Molecular and Cell Biology, Univ.of California, Berkeley, CA 94720 3Department of Bacteriology, University of Wisconsin, Madison, WI 53706 |
Distinct Functional States of E.coli
RNA Polymerase Affect its Pausing and
Elongation: A Single Molecules Study |
Braslavsky Ido
Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel febras@wicc.weizmann.ac.il www.weizmann.ac.il/~febras |
Measurments of single molecule enzymatic activity.
I am looking for a postdoctoral position |
Stretching and probing of single protein molecules: A study in structure-function relationship |
THEMES AND MAJOR OBJECTIVES OF THE WORKSHOP
In the past 2-3 years a flurry of novel techniques and methods (optical and magnetic tweezers, two-photon excitation , fluorescence correlation spectroscopy, evanescent wave fluorescence microscopy, near field scanning probe microscopy, etc.) have been applied to the manipulation, visualization and study of single bio-molecules, thereby opening a new vista on the investigation of biological processes at their most fundamental level. As a representative but non-exhaustive list, let us cite :
1) The studies of Steve Chu's group
on the relaxation dynamics of single DNA molecules (S.R.Quake. et
al., Nature 388, 151 (1997)).
2) The observation of novel structures
in overstitched DNA and their possible relevance to DNA/protein interactions
(in particular RecA) (P.Cluzel et al., Science 271, 792 (1996) and S.Smith
et al., Science 271, 795 (1996)).
3) The characterization of single
molecular motors, in particular myosin moving on actin or kinesin
(dynein) moving on microtubules (T.Funatsu et al., Nature 374, 555 (1995)
and M.J.Schnitzer & S.M.Block, Nature 388, 386 (1997)).
4) The investigation by the groups
of S.Block and J.Gelles of yet an other fundamental motor : a single
RNA-polymerase moving along a single DNA molecule (H.Yin et al., Science
270, 1653 (1995)).
5) The observation and study by
the group of Kinosita of the rotation of the F1-ATPase rotor (H.Noji
et al., Nature 386, 295 (1997)).
6) The measurement of the interaction
force between a single molecule and its ligand (E.-L. Florin et al.,
Science 264, 415 (1994)).
7) The observation of conformational
transitions on single molecules in solution (L.Edman et al., PNAS
93, 6710 (1996)).
8) The study of the fluorescence
emission by single molecules, in particular the biologically relevant
GFP (D.A. Vanden Bout et al., Science 277, 1074 (1997) & R.M. Dickson
et al. , Nature 388, 355 (1997)).
9) The observation of the quantal
release of neuro transmitters by a single synaptic vesicle (J.A.Steyer
et al., Nature 388, 474 (1997) & T.A.Ryan et al., Nature 388, 478 (1997)).
As evidenced by this non-exhaustive list, Single Molecule Biophysics is a very active and exciting field. It requires an interdisciplinary mastery of a number of state of the art techniques and methods. From the physics side one needs to perform micro-manipulation on single molecules with optical or magnetic tweezers or some adapted near field probes. One also needs to visualize single molecules and study their fluorescence spectra. Hence the need to master new techniques such as two photon fluorescence excitation, evanescent wave illumination, fluorescence correlation spectroscopy and low noise/low background photon detection. From the biological side one needs to have full control and characterization of the bio-molecules under study (protein purification and characterization (enzymatic activity, crystallography, NMR, etc.), site directed mutagenesis, protein fusions, etc.) . Finally from a chemical point of view there is a strong incentive to develop new reactive groups to label bio-molecules of interest at specific places.
This large array of techniques is used to address a number of fundamental problems.
1) To study the function of bio-molecules
by manipulating them individually, for example measuring the force
required to stall a single RNA-polymerase on its DNA tracks (see example
4 in the preceding list).
2) To study and induce structural
transitions in bio-molecules as exemplified in the DNA studies mentioned
previously (example 2 above).
3) To increase the spatial and
temporal resolution in the study of biological processes. In that respect
the measurement of the step size of myosin and of F1-ATPase and of the
number of hydrolyzed ATP per step are exemplary (examples 3 and 5 above).
4) To see how the thermodynamic
properties commonly measured on a large molecular population reflect
the observed behavior of a single molecule. Here the work of Gaub relating
the binding force of a single biotin/streptavidin link to the thermodynamic
enthalpy raises fascinating questions.
5) The theoretical description
of these molecular « engines » requires new concepts (e.g.
thermal ratchets) which differ radically from the ones used to study macroscopic
engines (e.g. Carnot cycle). The work of J.Prost and his co-workers is
a nice example of this new approach.
To justify our choice let us explain the reasons : when we started organising this workshop -which is the first real one concerning Single Molecule Biophysics- we thought that this was an emerging field and that a one hundred people workshop was the right scale (it is also the EMBO scale). As soon as we started receiving the preregistration forms it appeared that the community was larger than expected. We tried to see if it was possible to increase the number of participants. The main limitation comes from the lecture room. Other lecture rooms were proposed in Tours. Unfortunately, when we visited them they appeared to be either not large enough or too far from "La Croix Montoire" where we had already reservations.
We were thus left with the difficult choice of selecting one participant for every two applicants. As explained above, this evaluation was made mainly by the speakers. It is clear that the preregistration form has played a major role in this process. The overall level of the applicants was very high, and the fact that you are not selected does not mean anything regarding your work. It is clear that many applicants starting their activity in the field of single molecule biophysics have not been selected only because we have underestimated the size of the community. If there is another SMB conference later, it should be scale properly so that basically all applicants could participate.
This also means that if you have
been selected as participant and cannot come to a part or the entire workshop,
please consider giving your position
to someone on the waiting list.
Meanwhile we propose to open the conference for exchange between the people in the community : we shall post at the conference inquiries of applicants looking for postdoc, collaborations infos etc. In this idea, you can send us a contact page that we shall link to this page.
Depending on Tours facilities, we are thinking of placing transparencies on the web so that you may acces them.